ELECTRON MICROSCOPY

Sample

Mammalian AP-3 complex assembled on tubular membranes

Specimen Preperation
Sample Aggregation State HELICAL ARRAY
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE-PROPANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SUBTOMOGRAM AVERAGING
Number of Particles 5678
Reported Resolution (Å) 8.5
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details Two copies of previously modeled C1 coordinates of AP3 bound by ARF1 dimers was rigid body docked into the ARF1 centered reconstruction using UCSF ChimeraX. Duplicate ARF1 dimers were deleted.
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 3
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 3000
Maximum Defocus (nm) 6000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
VOLUME SELECTION UCSF Chimera ?
VOLUME SELECTION subTOM ?
VOLUME SELECTION Warp ?
VOLUME SELECTION M ?
VOLUME SELECTION RELION ?
CTF CORRECTION Warp ?
MODEL FITTING UCSF ChimeraX ?
FINAL EULER ASSIGNMENT RELION ?
CLASSIFICATION RELION ?
RECONSTRUCTION RELION ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION 3DCTF in WARP
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