ELECTRON MICROSCOPY

Sample

protein-specific O-mannosyl-transferase (TMEM260) incubated with Synthetic peptide derived from PLXNB2 IPT1 domain

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 271859
Reported Resolution (Å) 3.06
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 49.8
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 500
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model OTHER
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v4.7.0
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC v4.7.0
MODEL FITTING UCSF Chimera ?
MODEL FITTING PHENIX ?
MODEL FITTING Coot ?
INITIAL EULER ASSIGNMENT cryoSPARC v4.7.0
FINAL EULER ASSIGNMENT cryoSPARC v4.7.0
CLASSIFICATION cryoSPARC v4.7.0
RECONSTRUCTION cryoSPARC v4.7.0
MODEL REFINEMENT PHENIX ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
Feedback Form
Name
Email
Institute
Feedback