ELECTRON MICROSCOPY

Sample

Quinpirole-bound dopamine D3 receptor in complex with Gi protein

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument ?
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 191116
Reported Resolution (Å) 3.24
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 70
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 130000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details The original data (e.g., STAR files or micrograph metadata) are not available for this dataset. Therefore, the reported minimum and maximum defocus values (800 nm and 2500 nm, respectively) are based on standard cryo-EM data collection practices for similar resolution and instrumentation at the time of acquisition.
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC ?
CTF CORRECTION cryoSPARC ?
INITIAL EULER ASSIGNMENT cryoSPARC ?
CLASSIFICATION RELION 5.0
RECONSTRUCTION RELION 5.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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