ELECTRON MICROSCOPY

Sample

Escherichia coli 70S ribosome bound to mRNA, P-site fMet-tRNAfMet, glycyl-tRNAGly in A/T conformation, EF-Tu-GDPCP, and bottromycin

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument LEICA EM GP
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 443440
Reported Resolution (Å) 1.99
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 39.238
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 500
Maximum Defocus (nm) 1800
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.7.0
IMAGE ACQUISITION SerialEM ?
MASKING cryoSPARC 4.7.0
CTF CORRECTION cryoSPARC 4.7.0
MODEL FITTING UCSF Chimera 1.17.3
MODEL REFINEMENT PHENIX 1.19.2.4158
INITIAL EULER ASSIGNMENT cryoSPARC 4.7.0
FINAL EULER ASSIGNMENT cryoSPARC 4.7.0
CLASSIFICATION cryoSPARC 4.7.0
RECONSTRUCTION cryoSPARC 4.7.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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