ELECTRON MICROSCOPY

Sample

Cryo-EM Structure of designed Orb2 amyloid (LVLVF; Polymorph 2)

Specimen Preperation
Sample Aggregation State FILAMENT
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Vitrification Carried out in liquid Nitrogen atmosphere
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 94083
Reported Resolution (Å) 3.3
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol AB INITIO MODEL
Refinement Target Cross Correlation Coefficient
Overall B Value 3878
Fitting Procedure ?
Details Initial model fitting was performed using Coot and Phenix, with final adjustments completed in ChimeraX.
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 60
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2400
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 5.0-Beta-1
IMAGE ACQUISITION SerialEM 4.0
CTF CORRECTION CTFFIND ?
MODEL FITTING Coot 0.9.8.96
INITIAL EULER ASSIGNMENT RELION 5.0-beta-1-commit-b75b38
FINAL EULER ASSIGNMENT RELION 5.0-beta-1-commit-b75b38
CLASSIFICATION RELION 5.0-beta-1-commit-b75b38
RECONSTRUCTION RELION 5.0-beta-1-commit-b75b38
MODEL REFINEMENT PHENIX 1.21
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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