ELECTRON MICROSCOPY

Sample

vanadium-dependent haloperoxidase from Enhygromyxa salina bound to vanadate, bromide, and hydrogen peroxide

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details 1.5 sec blotting time
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 2144678
Reported Resolution (Å) 2.4
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 50
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2800
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cisTEM ?
CTF CORRECTION cisTEM ?
MODEL FITTING Coot 0.9.8.92
MODEL REFINEMENT PHENIX dev_5246
INITIAL EULER ASSIGNMENT cisTEM ?
FINAL EULER ASSIGNMENT cisTEM ?
CLASSIFICATION cisTEM ?
RECONSTRUCTION cisTEM ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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