ELECTRON MICROSCOPY

Sample

FlaA1 region of the outer sheath (located at the outer curvature) of thick flagellar filaments purified from planktonic T. denticola

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument LEICA EM GP
Cryogen Name ETHANE
Sample Vitrification Details The sample was back blotted
3D Reconstruction
Reconstruction Method HELICAL
Number of Particles 56413
Reported Resolution (Å) 2.7
Resolution Method FSC 0.143 CUT-OFF
Other Details This is reconstructed from pseudo-particles following symmetry expansion of 56413 particles by 6 assymmetric units with relion_particle_symmetry_expand: Twist of -33.57 and rise of 23.9.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol OTHER
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ModelAngelo automated the model builder was used to generate the initial model for refinement. This first given the full proteome for T.denticola and subsequently a fasta file containing the amino acids for only the relevant proteins.
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 57.7
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 500
Maximum Defocus (nm) 1800
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION crYOLO 1.9.9
MODEL REFINEMENT PHENIX 1.21.2_5419
RECONSTRUCTION RELION 5.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
NONE ?
Feedback Form
Name
Email
Institute
Feedback