ISDA: 9P3H

X-RAY DIFFRACTION

Crystallization Details

Method	pH	Temprature	Details
X-RAY DIFFRACTION	7.5	292	8.9 mM protein, 0.5 M sodium chloride, 0.01 M Tris-HCl, pH 8.3 against PEG's II (F3) [0.1 M HEPES, pH 7.5, 20% w/v PEG4000, 10% w/v isopropanol], cryoprotectant: 0.1 M HEPES, pH 7.5, 20% w/v PEG4000, 10% w/v isopropanol

Unit Cell:

a: 62.399 Å b: 144.741 Å c: 79.038 Å α: 90.00° β: 113.16° γ: 90.00°

Symmetry:

Space Group: P 1 21 1

Crystal Properties:

Matthew's Coefficient: 1.66 Solvent Content: 25.92

Refinement Statistics

Diffraction ID	Structure Solution Method	Cross Validation Method	Resolution Limit (High)	Resolution Limit (Low)	Number of Reflections (Observed)	Number of Reflections (R-free)	Percent Reflections (Observed)	R-Work	R-Free	Mean Isotropic
X-RAY DIFFRACTION	SAD	THROUGHOUT	1.85	30.00	95195	4978	91.10	0.19359	0.21491	24.458

Data Collection

Overall

Resolution Limit (High)	Resolution Limit (Low)	Percent Possible (Observed)	R Merge I (Observed)	R Sym I (Observed)	Net I Over Average Sigma (I)	Redundancy	Number Reflections (All)	Number Reflections (Observed)	Observed Criterion Sigma (F)	Observed Criterion Sigma (I)	B (Isotropic) From Wilson Plot
1.85	30.00	98.0	0.117	0.117	16.9	6.8	?	107367	?	-3.0	19.9

Highest Resolution Shell

#	Resolution Limits (Low)	Resolution Limits (High)	Percent Possible (All)	Percent Possible (Observed)	R-Sym I (Observed)	Mean I Over Sigma (Observed)	Redundancy	Number Unique Reflections (All)
1	1.85	1.88	97.6	?	?	2.2	6.7	?

Diffraction

Diffraction experiment

Crystal ID	Scattering Type	Data Collection Temprature	Detector	Detector Type	Details	Collection Date	Monochromator	Protocol
1		100 K			?

Radiation Source

Source	Type	Wavelength List	Synchrotron Site	Beamline
SYNCHROTRON	APS BEAMLINE 21-ID-D	1.12706	APS	21-ID-D

Software