ELECTRON MICROSCOPY

Sample

full-length p85 alpha and p110 alpha heterodimer/KRas complex bound to POPC/POPS/PIP2-MSP1E3D1 nanodiscs

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 223376
Reported Resolution (Å) 5.0
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 47.7
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.3.1
CTF CORRECTION cryoSPARC 4.3.1
MODEL FITTING UCSF ChimeraX 1.7.1
MODEL FITTING Rosetta 3.0
MODEL REFINEMENT Coot 8.0
MODEL REFINEMENT ISOLDE ?
INITIAL EULER ASSIGNMENT cryoSPARC 4.3.1
FINAL EULER ASSIGNMENT cryoSPARC 4.3.1
RECONSTRUCTION cryoSPARC 4.3.1
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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