ELECTRON MICROSCOPY

Sample

Structure of the human mitochondrial transcription initiation transitional complex, TC8

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 105850
Reported Resolution (Å) 2.69
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target Cross-correlation Coefficient
Overall B Value ?
Fitting Procedure ?
Details Initial docking of the starting model was done in Chimera and flexible/refined fitting done with Coot, and Phenix Real-Space Refinement.
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 45.44
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 400
Maximum Defocus (nm) 3200
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 165000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details Preliminary grid screening performed manually using TFS Glacios.
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.6.0
IMAGE ACQUISITION Leginon ?
CTF CORRECTION cryoSPARC 4.6.0
MODEL FITTING Coot 0.9.8.2
MODEL REFINEMENT PHENIX 1.21rc1_5015
INITIAL EULER ASSIGNMENT cryoSPARC 4.6.0
FINAL EULER ASSIGNMENT cryoSPARC 4.6.0
CLASSIFICATION cryoSPARC 4.6.0
RECONSTRUCTION cryoSPARC 4.6.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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