X-RAY DIFFRACTION

Crystallization Details
Method pH Temprature Details
X-RAY DIFFRACTION 6.0 293 20 mM potassium phosphate, pH 6.0, 1 M NaCl; 1.9 M ammonium sulfate
Unit Cell:
a: 65.891 Å b: 65.891 Å c: 40.685 Å α: 90.000° β: 90.000° γ: 120.000°
Symmetry:
Space Group: P 63
Crystal Properties:
Matthew's Coefficient: 1.78 Solvent Content: 31.03
Refinement Statistics
Diffraction ID Structure Solution Method Cross Validation Method Resolution Limit (High) Resolution Limit (Low) Number of Reflections (Observed) Number of Reflections (R-free) Percent Reflections (Observed) R-Work R-Free Mean Isotropic
X-RAY DIFFRACTION MOLECULAR REPLACEMENT THROUGHOUT 0.8500 23.3600 88220 4383 99.8500 0.1243 0.1333 10.0648
Data Collection
Overall
Resolution Limit (High) Resolution Limit (Low) Percent Possible (Observed) R Merge I (Observed) R Sym I (Observed) Net I Over Average Sigma (I) Redundancy Number Reflections (All) Number Reflections (Observed) Observed Criterion Sigma (F) Observed Criterion Sigma (I) B (Isotropic) From Wilson Plot
0.850 23.360 99.900 0.077 ? 17.760 16.274 ? 88292 ? ? 6.260
Highest Resolution Shell
# Resolution Limits (Low) Resolution Limits (High) Percent Possible (All) Percent Possible (Observed) R-Sym I (Observed) Mean I Over Sigma (Observed) Redundancy Number Unique Reflections (All)
1 0.850 0.870 99.400 ? ? 1.200 10.100 ?
Diffraction
Diffraction experiment
Crystal ID Scattering Type Data Collection Temprature Detector Detector Type Details Collection Date Monochromator Protocol
1 100 K ?
Radiation Source
Source Type Wavelength List Synchrotron Site Beamline
SYNCHROTRON SSRL BEAMLINE BL12-2 0.72929 SSRL BL12-2
Software
Software Name Purpose Version
XSCALE data scaling .
PHENIX refinement 1.13rc2_2986
PDB_EXTRACT data extraction 3.25
XDS data reduction .
PHENIX phasing .
Feedback Form
Name
Email
Institute
Feedback