ELECTRON MICROSCOPY

Sample

Influenza B (Panama/45) polymerase Hetermotrimer in complex with 3'5' cRNA promoter

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details blot for 3.5 sec before plunging
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 1012085
Reported Resolution (Å) 4.1
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value 57
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å2) 1.1
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model FEI TITAN KRIOS
Minimum Defocus (nm) 1300
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 130000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
IMAGE ACQUISITION EPU ?
CTF CORRECTION Gctf 1.18
MODEL FITTING UCSF Chimera 1.13
INITIAL EULER ASSIGNMENT cryoSPARC 2.5
FINAL EULER ASSIGNMENT cryoSPARC 2.5
CLASSIFICATION cryoSPARC 2.5
RECONSTRUCTION cryoSPARC 2.5
MODEL REFINEMENT PHENIX 1.14
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
NONE ?
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