ELECTRON MICROSCOPY

Sample

Human LRP6 ectodomain complexed with full length of Dkk1

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument
Cryogen Name
Sample Vitrification Details
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 5390
Reported Resolution (Å) 21
Resolution Method FSC 0.5 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type TVIPS TEMCAM-F224 (2k x 2k)
Electron Dose (electrons/Å2) 20
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model HITACHI H-9500SD
Minimum Defocus (nm) ?
Maximum Defocus (nm) ?
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.8
Imaging Mode BRIGHT FIELD
Specimen Holder Model SIDE ENTRY, EUCENTRIC
Nominal Magnification 80000
Calibrated Magnification ?
Source LAB6
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION SPIDER & WEB 17
IMAGE ACQUISITION EM-Menu 4
CTF CORRECTION SPIDER ?
MODEL FITTING Chimera 1.10.1
INITIAL EULER ASSIGNMENT SPIDER ?
FINAL EULER ASSIGNMENT SPIDER ?
CLASSIFICATION SPIDER ?
RECONSTRUCTION SPIDER ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION This was not done for RCT but for following single-particle reconstruction
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