ELECTRON MICROSCOPY

Sample

RNA-FREE EXO10-SKI7

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 25000
Reported Resolution (Å) 4.2
Resolution Method ?
Other Details SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3366. (DEPOSITION ID: 14342).
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID
Refinement Space
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector Type GATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å2) 21
Imaging Experiment
Date of Experiment 2014-10-20
Temprature (Kelvin)
Microscope Model FEI TITAN KRIOS
Minimum Defocus (nm) 1500
Maximum Defocus (nm) 3500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model .
Nominal Magnification 22500
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
RECONSTRUCTION EMAN 2
RECONSTRUCTION IMAGIC ?
RECONSTRUCTION RELION 1.3
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
? MICROGRAPHS
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