ELECTRON MICROSCOPY

Sample

BOVINE POL II ELONGATION COMPLEX

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 264134
Reported Resolution (Å) 3.4
Resolution Method ?
Other Details THE FOLLOWING REGIONS WERE MODELED INTO THE UNSHARPENED EC1 MAP (EMDB-3218) DUE TO WEAKER DENSITY. RPB1 433-437, RPB1 1103-1108 (POLY-ALANINE), RPB1 1198-1205, RPB1 1262-1267, RPB1 1278-1281, RPB2 242-252, RPB2 326-331, RPB2 457-461 SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3218. (DEPOSITION ID: 13928).
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol OTHER
Refinement Target ?
Overall B Value 137
Fitting Procedure ?
Details REFINEMENT PROTOCOL--CRYO-EM
Data Acquisition
Detector Type GATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å2) 43
Imaging Experiment
Date of Experiment 2014-12-01
Temprature (Kelvin)
Microscope Model FEI TITAN KRIOS
Minimum Defocus (nm) 600
Maximum Defocus (nm) 3100
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2
Imaging Mode BRIGHT FIELD
Specimen Holder Model .
Nominal Magnification 37000
Calibrated Magnification 37037
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
? INDIVIDUAL PARTICLES
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