ELECTRON MICROSCOPY

Sample

GLUK2

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ETHANE
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 21360
Reported Resolution (Å) 7.6
Resolution Method ?
Other Details COORDINATES FROM 3KG2 WERE FIT AS SEVEN INDEPENDENT RIGID BODIES CONSISTING OF TWO ATD DIMERS FROM PDB ID 3H6G FOUR LBD MONOMERS FROM PDB 3G3F AND A TMD TETRAMER FROM PDB 3KG2 GEOMETRY AND STEREOCHEMISTRY OUTLIERS ARE THOSE PRESENT IN PDB 3H6G 3G3F AND 3KG2 USED FOR RIGID BODY FITS SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2685. (DEPOSITION ID: 12610).
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details METHOD--RIGID BODY REFINEMENT PROTOCOL--X-RAY
Data Acquisition
Detector Type FEI FALCON II (4k x 4k)
Electron Dose (electrons/Å2) 25
Imaging Experiment
Date of Experiment 2013-08-01
Temprature (Kelvin)
Microscope Model FEI TITAN KRIOS
Minimum Defocus (nm) 2000
Maximum Defocus (nm) 3500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model .
Nominal Magnification 47000
Calibrated Magnification 47000
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
MODEL FITTING UCSF Chimera ?
RECONSTRUCTION RELION ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
? EACH PARTICLE
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