ISDA: 4I0X

X-RAY DIFFRACTION

Crystallization Details

Method	pH	Temprature	Details
X-RAY DIFFRACTION	5.6	291	RESERVOIR: 0.1M tri-sodium citrate, pH5.6, 0.2M potassium sodium tartrate, 2M ammonium sulfate. PROTEIN CONCENTRATION: 11 MG/ML. PROTEIN BUFFER: 20 mM Tris, pH 8.0, 150mM NaCl, 10% Glycerol, 2 mM B-ME. CRYO: reservoir solution plus 25% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 291K

Unit Cell:

a: 47.600 Å b: 74.060 Å c: 84.420 Å α: 114.57° β: 103.28° γ: 95.52°

Symmetry:

Space Group: P 1

Crystal Properties:

Matthew's Coefficient: 2.02 Solvent Content: 39.02

Refinement Statistics

Diffraction ID	Structure Solution Method	Cross Validation Method	Resolution Limit (High)	Resolution Limit (Low)	Number of Reflections (Observed)	Number of Reflections (R-free)	Percent Reflections (Observed)	R-Work	R-Free	Mean Isotropic
X-RAY DIFFRACTION	MOLECULAR REPLACEMENT	?	1.956	19.995	67097	6709	93.37	0.1647	0.2131	?

Data Collection

Overall

Resolution Limit (High)	Resolution Limit (Low)	Percent Possible (Observed)	R Merge I (Observed)	R Sym I (Observed)	Net I Over Average Sigma (I)	Redundancy	Number Reflections (All)	Number Reflections (Observed)	Observed Criterion Sigma (F)	Observed Criterion Sigma (I)	B (Isotropic) From Wilson Plot
1.956	19.995	93	0.057	?	13.69	1.959	72126	67097	?	?	?

Highest Resolution Shell

#	Resolution Limits (Low)	Resolution Limits (High)	Percent Possible (All)	Percent Possible (Observed)	R-Sym I (Observed)	Mean I Over Sigma (Observed)	Redundancy	Number Unique Reflections (All)
1

Diffraction

Diffraction experiment

Crystal ID	Scattering Type	Data Collection Temprature	Detector	Detector Type	Details	Collection Date	Monochromator	Protocol
1		100 K

Radiation Source

Source	Type	Wavelength List	Synchrotron Site	Beamline
SYNCHROTRON	APS BEAMLINE 24-ID-C	0.9791	APS	24-ID-C

Software