ELECTRON MICROSCOPY

Sample

CMV-STALLED WHEAT GERM 80S-RNC BOUND TO ERF1 AND ABCE1-ADPNP

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details VITRIFICATION 1 -CRYOGEN- ETHANE, HUMIDITY- 100, INSTRUMENT- FEI VITROBOT MARK IV, METHOD- BLOT FOR 3 SECONDS BEFORE PLUNGING
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 39309
Reported Resolution (Å) 8.75
Resolution Method ?
Other Details SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2598.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details METHOD--RIGID BODY
Data Acquisition
Detector Type TVIPS TEMCAM-F416 (4k x 4k)
Electron Dose (electrons/Å2) 20
Imaging Experiment
Date of Experiment 2013-02-20
Temprature (Kelvin)
Microscope Model FEI MORGAGNI
Minimum Defocus (nm) ?
Maximum Defocus (nm) ?
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model .
Nominal Magnification ?
Calibrated Magnification 147136
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
MODEL FITTING Coot ?
MODEL FITTING MDFF ?
MODEL FITTING UCSF Chimera ?
PARTICLE SELECTION STARFISH ?
RECONSTRUCTION SPIDER ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
? ON VOLUMES (SPIDER)
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