SOLUTION NMR

NMR Experiment
Experiment Type Sample Contents Ionic Strength Solvent pH Pressure Temprature (K)
NMR Spectrometer Information
Spectrometer Manufacturer Model Field Strength
1 Bruker AVANCE 800
NMR Refinement
Method Details Software
ARIA THE STRUCTURE WAS DETERMINED USING A MULTI-STEP PROTOCOL. THE INITIAL COORDINATES OF THE ISOLATED DOMAINS WERE BASED ON PDB ID 3NMU BY XUE ET AL. 2010. MOL. CELL 39, 939-49. THE LINKERS BETWEEN PROTEIN DOMAINS AND RNA SECONDARY STRUCTURE ELEMENTS WERE LEFT FLEXIBLE. PRE-BASED DISTANCE RESTRAINT AND MULTIPLE SANS DATA WERE USED EITHER FOR STRUCTURE CALCULATION OR FOR STRUCTURE SELECTION. PRE-DERIVED DISTANCE RESTRAINTS WERE BASED ON PARAMAGNETIC RELAXATION ENHANCEMENT DATA FROM FIVE INDEPENDENT CYSTEINE MUTANTS (NOP5: E68C, E199C, D250C. L7AE: Q47C, K73C) COVALENTLY MODIFIED BY 3-(2-IODOACETAMIDO)-2,2,5,5, TETRAMETHYL-1- PYRROLIDINYLOXY RADICAL (IODOACETAMIDO-PROXYL). 1
NMR Ensemble Information
Conformer Selection Criteria ?
Conformers Calculated Total Number ?
Conformers Submitted Total Number 1
Representative Model ()
Computation: NMR Software
# Classification Version Software Name Author
1 refinement 1.2 CNS BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,READ, RICE,SIMONSON,WARREN
2 structure solution ? NMRView ?
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