ELECTRON MICROSCOPY

Sample

MecA-ClpC (E280A,E618A) with ATP

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 36688
Reported Resolution (Å) 10.0
Resolution Method FSC 0.5 CUT-OFF
Other Details (Single particle--Applied symmetry: C6)
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target Cross-correlation
Overall B Value ?
Fitting Procedure ?
Details REFINEMENT PROTOCOL--flexible DETAILS--Protocol- Initial local fitting was done using Chimera and then MDFF was used for flexible fitting. ref- Trabuco, L.G., Villa, E., Mitra, K., Frank, J. and Schulten, K. (2008) Flexible fitting of atomic structures into electron microscopy maps using molecular dynamics. Structure, 16, 673-683
Data Acquisition
Detector Type FEI EAGLE (4k x 4k)
Electron Dose (electrons/Å2) 20
Imaging Experiment
Date of Experiment 2010-09-09
Temprature (Kelvin)
Microscope Model FEI TITAN KRIOS
Minimum Defocus (nm) 1700
Maximum Defocus (nm) 4000
Minimum Tilt Angle (degrees) 0.0
Maximum Tilt Angle (degrees) 0.0
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 59000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
MODEL FITTING MDFF ?
RECONSTRUCTION SPIDER ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
. each defocus group on 3D level
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