ELECTRON MICROSCOPY

Sample

HIV-1 BaL

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK I
Cryogen Name ETHANE
Sample Vitrification Details Ethane 77K 100%RH Vitrobot
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles ?
Reported Resolution (Å) 20
Resolution Method ?
Other Details IMOD was used for tomographic reconstruction. Programs developed in-house were used for alignment and classification of individual tomographic volumes. The final map is the average of ~4000 individual spikes.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target correlation coefficient
Overall B Value ?
Fitting Procedure ?
Details METHOD--Automatic. The coordinates for this entry and the two related entries 3DNL and 3DNN are based on fitting 1GC1 coordinates for the gp120 monomer deposited previously by Kwong et al in 1998 into the density map for the trimer derived by electron microscopy. Therefore, authors did not deposit new structure factors, and, any features such as unusual torsion angles are the same as in the 1GC1 coordinates. REFINEMENT PROTOCOL--rigid body
Data Acquisition
Detector Type GENERIC CCD
Electron Dose (electrons/Å2) 80
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model FEI POLARA 300
Minimum Defocus (nm) 2000
Maximum Defocus (nm) 4000
Minimum Tilt Angle (degrees) -70
Maximum Tilt Angle (degrees) 70
Nominal CS 2.2
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 34000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
MODEL FITTING UCSF Chimera ?
RECONSTRUCTION IMOD ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
. No CTF correction applied
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