ELECTRON MICROSCOPY

Sample

T33-Fus-1B asymmetric unit - Designed tetrahedral protein cage based on helical fusion and machine learning

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE-PROPANE
Sample Vitrification Details blotting 3 seconds, blot force 0
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 2200000
Reported Resolution (Å) 3.90
Resolution Method FSC 0.143 CUT-OFF
Other Details The particles were expanded in T symmetry
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 40
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS GLACIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2200
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v5.0.4
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC v5.0.4
MODEL FITTING UCSF ChimeraX 1.11.1
MODEL REFINEMENT PHENIX 1.21.2_5419
INITIAL EULER ASSIGNMENT cryoSPARC v5.0.4
FINAL EULER ASSIGNMENT cryoSPARC v5.0.4
CLASSIFICATION cryoSPARC v5.0.4
RECONSTRUCTION cryoSPARC v5.0.4
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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