ELECTRON MICROSCOPY

Sample

T33-Fus-1A asymmetric unit - Designed tetrahedral protein cage based on helical fusion and machine learning

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE-PROPANE
Sample Vitrification Details blotting 3 seconds, blot force 0
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 435000
Reported Resolution (Å) 3.00
Resolution Method FSC 0.143 CUT-OFF
Other Details The particles were symmetry expanded in T
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 40
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS GLACIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2200
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v5.0.4
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC v5.0.4
MODEL FITTING UCSF ChimeraX 1.11.1
INITIAL EULER ASSIGNMENT cryoSPARC v5.0.4
FINAL EULER ASSIGNMENT cryoSPARC v5.0.4
CLASSIFICATION cryoSPARC v5.0.4
RECONSTRUCTION cryoSPARC v5.0.4
MODEL REFINEMENT PHENIX 1.21.2_5419
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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