ELECTRON MICROSCOPY

Sample

Octameric complex of K4 containing mutated histidines in the HKD motif, with DNA bound in chains A and D

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 23730
Reported Resolution (Å) 2.60
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target cross-correlation coefficient
Overall B Value 133.56
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 42
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 700
Maximum Defocus (nm) 2400
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC 4
MODEL FITTING UCSF ChimeraX ?
MODEL FITTING Coot ?
INITIAL EULER ASSIGNMENT cryoSPARC 4
FINAL EULER ASSIGNMENT cryoSPARC 4
CLASSIFICATION cryoSPARC 4
RECONSTRUCTION cryoSPARC 4
MODEL REFINEMENT PHENIX 2.0_5936
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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