SOLUTION NMR

NMR Experiment
Experiment Type Sample Contents Ionic Strength Solvent pH Pressure Temprature (K)
1 3D 1H-15N NOESY 1.0mM [U-99% 13C; U-99% 15N] aFGF-1, 500mM NDSB-new-2, 25mM sodium phosphate-3, 150mM NaCl-4, 1mM dioxane-5, 90% H2O/10% D2O 190 90% H2O/10% D2O 6.6 ambient 298
2 3D 1H-13C NOESY 1.0mM [U-99% 13C; U-99% 15N] aFGF-1, 500mM NDSB-new-2, 25mM sodium phosphate-3, 150mM NaCl-4, 1mM dioxane-5, 90% H2O/10% D2O 190 90% H2O/10% D2O 6.6 ambient 298
NMR Spectrometer Information
Spectrometer Manufacturer Model Field Strength
1 Bruker AVANCE 800
NMR Refinement
Method Details Software
torsion angle dynamics ? 1
NMR Ensemble Information
Conformer Selection Criteria target function
Conformers Calculated Total Number 100
Conformers Submitted Total Number 20
Representative Model 1 (lowest energy)
Computation: NMR Software
# Classification Version Software Name Author
1 structure solution 1.0b CYANA Guntert, Mumenthaler and Wuthrich
2 processing ? NMRPipe Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
3 collection ? XwinNMR Bruker Biospin
4 data analysis 5.0.4 NMRView Johnson, One Moon Scientific
5 data analysis 0.9820 KUJIRA Kobayashi, N.
6 refinement 1.0b CYANA Guntert, Mumenthaler and Wuthrich
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