ISDA: 2GA5

SOLUTION NMR

NMR Experiment

Experiment	Type	Sample Contents	Ionic Strength	Solvent	pH	Pressure	Temprature (K)
1	NOESY	1 mM protein	?	25 mM phosphate buffer (pH 7) 10 mM deuterium oxide/90% water	7	AMBIENT	298
2	3D_15N-separated_NOESY	1 mM protein	?	25 mM phosphate buffer (pH 7) 10 mM deuterium oxide/90% water	7	AMBIENT	298
3	4D_13C-separated_NOESY	1 mM protein	?	25 mM phosphate buffer (pH 7) 10 mM deuterium oxide/90% water	7	AMBIENT	298
4	4D_13C/15N-separated_NOESY	1 mM protein	?	25 mM phosphate buffer (pH 7) 10 mM deuterium oxide/90% water	7	AMBIENT	298

NMR Spectrometer Information

Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	600

NMR Refinement

Method	Details	Software
torsion angle dynamics	?	1

NMR Ensemble Information

Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software

#	Classification	Version	Software Name	Author
1	refinement	2.1	CYANA	P.GUNTERT ET AL.
2	structure solution	2.1	CYANA	?