ELECTRON MICROSCOPY

Sample

Tobacco mosaic virus

Specimen Preperation
Sample Aggregation State HELICAL ARRAY
Vitrification Instrument ?
Cryogen Name ETHANE
Sample Vitrification Details Sample prepared on the cryoWriter.
3D Reconstruction
Reconstruction Method HELICAL
Number of Particles 880163
Reported Resolution (Å) 1.71
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type HELICAL
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details Initial rigid-body fit done on ChimeraX. Real-space refinement done in Phenix.
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 45
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.6
IMAGE ACQUISITION EPU ?
CTF CORRECTION cryoSPARC 4.6
MODEL FITTING UCSF ChimeraX 1.11
INITIAL EULER ASSIGNMENT cryoSPARC 4.6
FINAL EULER ASSIGNMENT cryoSPARC 4.6
RECONSTRUCTION cryoSPARC 4.6
MODEL REFINEMENT PHENIX 2.0
MODEL REFINEMENT Coot 1.1.15
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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