ELECTRON MICROSCOPY

Sample

14 subunit helical assembly of cyanide dihydratase from Stutzerimonas stutzeri (Pseudomonas stutzeri AK1)

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument ?
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 857344
Reported Resolution (Å) 3.07
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol AB INITIO MODEL
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOCONTINUUM (6k x 4k)
Electron Dose (electrons/Å2) 1.2
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1500
Maximum Defocus (nm) 3000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC ?
CTF CORRECTION cryoSPARC ?
MODEL FITTING PHENIX ?
MODEL REFINEMENT PHENIX ?
MODEL FITTING ISOLDE ?
MODEL REFINEMENT ISOLDE ?
RECONSTRUCTION cryoSPARC ?
PARTICLE SELECTION cryoSPARC ?
RECONSTRUCTION cryoSPARC ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
Feedback Form
Name
Email
Institute
Feedback