ELECTRON MICROSCOPY

Sample

Complex of translocation and assembly module subunit A (TamA) with translocation and assembly module subuni B DUF490 (TamB DUF490).

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details 3 uL sample applied for blotting. Wait time of 60s, blot time of 3s with a blot force of 9.
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 103937
Reported Resolution (Å) 3.71
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 58
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 200
Maximum Defocus (nm) 2300
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.7.0
MODEL REFINEMENT PHENIX 1.21.2_5419
CTF CORRECTION cryoSPARC 4.7.0
RECONSTRUCTION cryoSPARC 4.7.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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