SOLUTION NMR

NMR Experiment
Experiment Type Sample Contents Ionic Strength Solvent pH Pressure Temprature (K)
1 3D_13C-separated_NOESY 0.66mM cNMP-binding domain U-15N, 13C; 20mM d-Tris-HCl(pH 7.5); 200mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O 220mM 90% H2O/10% D2O 7.5 ambient 298
2 3D_15N-separated_NOESY 0.66mM cNMP-binding domain U-15N, 13C; 20mM d-Tris-HCl(pH 7.5); 200mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O 220mM 90% H2O/10% D2O 7.5 ambient 298
NMR Spectrometer Information
Spectrometer Manufacturer Model Field Strength
1 Bruker AVANCE 600
NMR Refinement
Method Details Software
torsion angle dynamics ? 1
NMR Ensemble Information
Conformer Selection Criteria structures with the least restraint violations, structures with the lowest energy, target function
Conformers Calculated Total Number 100
Conformers Submitted Total Number 20
Representative Model ()
Computation: NMR Software
# Classification Version Software Name Author
1 collection 2.6 XwinNMR Bruker
2 processing 20030801 NMRPipe Delaglio, F.
3 data analysis 5.0.4 NMRView Johnson, B.A.
4 data analysis 0.901 KUJIRA Kobayashi, N.
5 structure solution 2.0.17 CYANA Guentert, P.
6 refinement 2.0.17 CYANA Guentert, P.
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