SOLUTION NMR

NMR Experiment
Experiment Type Sample Contents Ionic Strength Solvent pH Pressure Temprature (K)
1 3D_15N-separated_NOESY 1.12mM IscU domain U-15N, 13C; 20mM d-Tris-HCl; 100mM NaCl; 10mM d-DTT; 0.02% NaN3; 0.01mM ZnCl2; 90% H2O, 10% D2O 120mM 90% H2O/10% D2O 7.0 ambient 298
2 3D_13C-separated_NOESY 1.12mM IscU domain U-15N, 13C; 20mM d-Tris-HCl; 100mM NaCl; 10mM d-DTT; 0.02% NaN3; 0.01mM ZnCl2; 90% H2O, 10% D2O 120mM 90% H2O/10% D2O 7.0 ambient 298
NMR Spectrometer Information
Spectrometer Manufacturer Model Field Strength
1 Bruker AVANCE 800
NMR Refinement
Method Details Software
NMR Ensemble Information
Conformer Selection Criteria structures with the least restraint violations, target function
Conformers Calculated Total Number 100
Conformers Submitted Total Number 20
Representative Model 1 (lowest energy)
Computation: NMR Software
# Classification Version Software Name Author
1 collection 2.6 XwinNMR Bruker
2 processing 20030801 NMRPipe Delaglio, F.
3 data analysis 5.0.4 NMRView Johnson, B.A.
4 data analysis 0.870 KUJIRA Kobayashi, N.
5 structure solution 2.0.17 CYANA Guntert, P.
6 refinement 2.0.17 CYANA Guntert, P.
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