SOLUTION NMR

NMR Experiment
Experiment Type Sample Contents Ionic Strength Solvent pH Pressure Temprature (K)
1 3D_13C-separated_NOESY 1.2MM U-13C, 15N BOLA-LIKE PROTEIN; 50MM SODIUM PHOSPHATE BUFFER, 100MM SODIUM CHLORIDE, 0.02% SODIUM AZIDE, 1MM D-DTT; 90% H2O, 10% D2O 100mM 90% H2O/10% D2O 6.0 ambient 298
2 3D_15N-separated_NOESY 1.2MM U-13C, 15N BOLA-LIKE PROTEIN; 50MM SODIUM PHOSPHATE BUFFER, 100MM SODIUM CHLORIDE, 0.02% SODIUM AZIDE, 1MM D-DTT; 90% H2O, 10% D2O 100mM 90% H2O/10% D2O 6.0 ambient 298
NMR Spectrometer Information
Spectrometer Manufacturer Model Field Strength
1 Bruker AVANCE 600
NMR Refinement
Method Details Software
Simulated annealing, Torsion angle dynamics ? 1
NMR Ensemble Information
Conformer Selection Criteria target function
Conformers Calculated Total Number 200
Conformers Submitted Total Number 20
Representative Model 1 (lowest energy)
Computation: NMR Software
# Classification Version Software Name Author
1 data analysis 1.8 NMRPipe Delaglio
2 refinement 1.0 CNS ?
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