SOLUTION NMR

NMR Experiment
Experiment Type Sample Contents Ionic Strength Solvent pH Pressure Temprature (K)
1 3D_13C-separated_NOESY 0.66 mM DnaK(393-507); 3.3 mM NRLLLTG; 10 mM sodium phosphate buffer, pH 7.4 10 mM sodium phosphate 90% H2O/10% D2O 7.4 ambient 303
2 3D_15N-separated_NOESY 0.66 mM DnaK(393-507); 3.3 mM NRLLLTG; 10 mM sodium phosphate buffer, pH 7.4 10 mM sodium phosphate 90% H2O/10% D2O 7.4 ambient 303
3 H(C)CH_TOCSY 0.66 mM DnaK(393-507); 3.3 mM NRLLLTG; 10 mM sodium phosphate buffer, pH 7.4 10 mM sodium phosphate 90% H2O/10% D2O 7.4 ambient 303
4 (H)CCH_TOCSY 0.66 mM DnaK(393-507); 3.3 mM NRLLLTG; 10 mM sodium phosphate buffer, pH 7.4 10 mM sodium phosphate 90% H2O/10% D2O 7.4 ambient 303
5 15N FILTERED/EDITED NOESY 0.66 mM DnaK(393-507); 3.3 mM NRLLLTG; 10 mM sodium phosphate buffer, pH 7.4 10 mM sodium phosphate 90% H2O/10% D2O 7.4 ambient 303
6 13C FILTERED/EDITED NOESY 0.66 mM DnaK(393-507); 3.3 mM NRLLLTG; 10 mM sodium phosphate buffer, pH 7.4 10 mM sodium phosphate 90% H2O/10% D2O 7.4 ambient 303
NMR Spectrometer Information
Spectrometer Manufacturer Model Field Strength
NMR Refinement
Method Details Software
torsion angle dynamics ? 1
NMR Ensemble Information
Conformer Selection Criteria structures with acceptable covalent geometry,structures with the least restraint violations,structures with the lowest energy
Conformers Calculated Total Number 60
Conformers Submitted Total Number 15
Representative Model 1 (closest to the average)
Computation: NMR Software
# Classification Version Software Name Author
1 collection 2.6 VNMR varian
2 processing 2.6 VNMR varian
3 processing ? NMRPipe Delaglio
4 data analysis 3.2 XEASY Wuthrich
5 structure solution 1.0 ARIA/(CNS) Nilges
6 refinement 1.0 ARIA/(CNS) Nilges
Feedback Form
Name
Email
Institute
Feedback