ELECTRON MICROSCOPY

Sample

SKIN

Specimen Preperation
Sample Aggregation State TISSUE
Vitrification Instrument ?
Cryogen Name NITROGEN
Sample Vitrification Details high pressure frozen and freezing substituted with 1% OsO4/0.1% Ur-Ac. in acetone, LX-112 resin embeded sample. 50nm thin section
3D Reconstruction
Reconstruction Method TOMOGRAPHY
Number of Particles ?
Reported Resolution (Å) 30
Resolution Method ?
Other Details sectioned sample thickness 47.2 nm; +/- 75 degree dual-axis tilt-series with increment 1 degree, dual-axis electron tomography with IMOD. 1.169nm alignment error
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target best visual fit using the program AmiraMOL 3.0
Overall B Value ?
Fitting Procedure ?
Details METHOD--tracking 3D density REFINEMENT PROTOCOL--rigid body
Data Acquisition
Detector Type GENERIC GATAN
Electron Dose (electrons/Å2) 1200
Imaging Experiment
Date of Experiment 2002-11-23
Temprature (Kelvin)
Microscope Model FEI/PHILIPS CM200FEG
Minimum Defocus (nm) 300
Maximum Defocus (nm) 500
Minimum Tilt Angle (degrees) -78
Maximum Tilt Angle (degrees) 79
Nominal CS 2.0
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 50000
Calibrated Magnification 68276
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details Dose is for whole dataset, not individual images
Imaging Experiment
Task Software Package Version
RECONSTRUCTION IMOD ?
MODEL FITTING Amira 3
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
. no CTF correction. Imaging at underfocus 0.4 micron with CM200FEG microscope at 50,000 magnification
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