ELECTRON MICROSCOPY

Sample

Ternary complex of Human Argonaute2-miR200 guide with 13nt Target RNA (RISC-ZT13)in closed conformation

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument LEICA PLUNGER
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 239359
Reported Resolution (Å) 3.40
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol AB INITIO MODEL
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 75.5
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 700
Maximum Defocus (nm) 2200
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Warp v 1.0.9
IMAGE ACQUISITION EPU v 2.10.0.5
CTF CORRECTION Warp v 1.0.9
MODEL FITTING UCSF ChimeraX ?
MODEL REFINEMENT PHENIX 1.20.1_4487
INITIAL EULER ASSIGNMENT cryoSPARC v3.258
CLASSIFICATION cryoSPARC v3.258
RECONSTRUCTION cryoSPARC v3.258
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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