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IPD9272 |
From Omics to Function: NCU05627 Emerges as a Pivotal Sugar Transporter for Cellulase Secretion in Neurospora crassa |
Dr. Nidhi Adlakha |
This study investigates the role of sugar transporters in cellulase secretion and polysaccharide utilization in Neurospora crassa using an LC–MS/MS-based proteomics approach. Comparative proteomic profiling identified 2480 proteins shared between the experimental conditions, including several upregulated sugar transporters and carbohydrate-active enzymes (CAZymes) associated with biomass degradation. Functional annotation through Gene...
This study investigates the role of sugar transporters in cellulase secretion and polysaccharide utilization in Neurospora crassa using an LC–MS/MS-based proteomics approach. Comparative proteomic profiling identified 2480 proteins shared between the experimental conditions, including several upregulated sugar transporters and carbohydrate-active enzymes (CAZymes) associated with biomass degradation. Functional annotation through Gene Ontology analysis revealed enrichment of proteins involved in catalytic activity and metabolic processes. Among the identified sugar transporters, the putative xylose transporter NCU05627 was selected for further characterization due to its previously uncharacterized role in cellulase induction. Phylogenetic analysis grouped NCU05627 within the hexose transporter-enriched clade, suggesting a conserved role in sugar uptake. Functional studies demonstrated that deletion of NCU05627 reduced the secretion of plant cell wall-degrading enzymes. This dataset provides comprehensive insight into the sugar transportome of N. crassa and identifies transporter proteins potentially involved in cellulose degradation and fungal biomass conversion.
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DBT-Regional Centre for Biotechnology, Haryana, Faridabad, India |
Bottom-up |
2027-12-31 |
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