| 1 |
IPD3461 |
Identification of differentially regulated proteins of Caenorhabditis elegans during Salmonella enterica Serovar Typhi exposure using Mass Spectrometry |
Dr Krishnaswamy Balamurugan |
Recently, proteomics based studies have become the primary contributors for the understanding of host-pathogen interactions and discovery of various drug targets. Herewith, we have used the popular eukaryotic model organism Caenorhabditis elegans to study the host pathogen interactions at protein levels. Especially, we have employed proteomics approach to monitor the...
Recently, proteomics based studies have become the primary contributors for the understanding of host-pathogen interactions and discovery of various drug targets. Herewith, we have used the popular eukaryotic model organism Caenorhabditis elegans to study the host pathogen interactions at protein levels. Especially, we have employed proteomics approach to monitor the protein players that underwent for differential regulation during host-pathogen interaction through label-free quantitation LC-MS/MS technique. As a result of LC-MS/MS analysis, a total of 3747 differentially regulated proteins were identified during pathogenic condition among which 12 and 04 proteins were upregulated and downregulated with at least 2 folds, respectively. Bioinformatics analyses result suggested that the upregulated proteins have crucial roles in TCA cycle, whereas downregulated proteins were majorly involving in the N-glycosylation synthesis. Additionally, the mRNA levels of up and downregulated proteins were validated using qPCR analysis. Overall, the study suggested that the bacterial infection could target multiple molecular mechanisms to modulate the host machinery which favour the bacterial pathogen for their pathogenesis.
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Department of BiotechnologyAlagappa University, Karaikudi |
Shotgun proteomics |
2025-01-15 |
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| 2 |
IPD2357 |
Global proteomic analysis of changes in the Caenorhabditis elegans proteome during Klebsiella pneumoniae infection |
Dr Krishnaswamy Balamurugan |
In this study, the changes in the global proteomics of C. elegans were analyzed by proteomics approach. The nematodes were exposed to K. pneumoniae for different time-points (12, 24 and 36 h) and the changes in the regulation were analyzed by employing mass spectrometry.
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Department of BiotechnologyAlagappa University, Karaikudi |
Gel-based experiment |
2025-01-15 |
28932706
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| 3 |
IPD4220 |
Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair |
Professor Krishnaswamy Balamurugan |
Now a days, wound healing is becoming a global threat that impact economy of the country severely. Though the steps involved in wound healing are well characterised, direct therapeutics for the accelerated wound healing is comparatively less. This is solely because of the incomplete mechanism of healing and the lack...
Now a days, wound healing is becoming a global threat that impact economy of the country severely. Though the steps involved in wound healing are well characterised, direct therapeutics for the accelerated wound healing is comparatively less. This is solely because of the incomplete mechanism of healing and the lack of knowledge on molecular players involved in wound healing. Hence, in the present study, we have investigated the molecular players involved in wound healing process using a versatile model organism Caenorhabditis elegans through proteomic analyses. Especially, we have employed the high through put proteomic analyses tools such as 2-D GE and LCMS/MS analysis to uncover the molecular players involved in wound healing. As a result of LC-MS/MS analysis at 0 and 24 h, a total of 435 proteins were regulated in both unwounded and wounded conditions in which 13 and 10 proteins were significantly differential regulated. Bioinformatics analyses result suggested that molecular players involved in cortical actin cytoskeleton organization, protein phosphatase binding, and proton transporting ATP synthase activity were identified at 0 h; skeletal muscle myosin thick filament assembly, actin filament depolymerization were identified at 24 h.
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Department of BiotechnologyAlagappa University, Karaikudi |
Top-down |
2025-01-15 |
33831597
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| 4 |
IPD7793 |
Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair |
Professor Krishnaswamy Balamurugan |
Now a days, wound healing is becoming a global threat that impact economy of the country severely. Though the steps involved in wound healing are well characterised, direct therapeutics for the accelerated wound healing is comparatively less. This is solely because of the incomplete mechanism of healing and the lack...
Now a days, wound healing is becoming a global threat that impact economy of the country severely. Though the steps involved in wound healing are well characterised, direct therapeutics for the accelerated wound healing is comparatively less. This is solely because of the incomplete mechanism of healing and the lack of knowledge on molecular players involved in wound healing. Hence, in the present study, we have investigated the molecular players involved in wound healing process using a versatile model organism Caenorhabditis elegans through proteomic analyses. Especially, we have employed the high through put proteomic analyses tools such as 2-D GE and LCMS/MS analysis to uncover the molecular players involved in wound healing. As a result of 2-D GE analysis, a total of 35 differentially regulated proteins were identified during injury in which 22 and 13 proteins were upregulated and downregulated, respectively. Bioinformatics analyses result suggested that the upregulated proteins have crucial roles in serotonin/ acetyl choline synthesis and calcium signalling, whereas downregulated proteins were majorly involving in ubiquitin mediated proteolysis. Additionally, the mRNA levels of up and downregulated proteins were validated using qPCR analysis. Overall, the study suggested that injury initiates multiple molecular mechanisms to repair the damage.
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Department of BiotechnologyAlagappa University, Karaikudi |
Gel-based experiment |
2025-01-15 |
33831597
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| 5 |
IPD4029 |
p38-MAPK recruits the proteolysis pathways in Caenorhabditis elegans during bacterial infection |
Dr Krishnaswamy Balamurugan |
TiO2 column chromatography, MALDI-ToF-MS, SUMOylation, Coimmunoprecipitation and LC-MS/MS
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Department of BiotechnologyAlagappa University, Karaikudi |
Shotgun proteomics, Affinity purification coupled with mass spectrometry proteomics |
2025-01-15 |
35124023
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| 6 |
IPD2932 |
The salivary proteome profile in women throughout the menstrual cycle characterized by HR-LC-MS/MS |
Dr Govindaraju Archunan |
Generally saliva consists of mixture of proteins electrolytes and small organic compounds. This whole saliva stands for secretions from major (submandibular, sublingual and parotid) and minor salivary glands, along with crevicular fluid, bacteria and cellular debris. The secretion of saliva is regulated under the autonomic nervous system via signal transduction...
Generally saliva consists of mixture of proteins electrolytes and small organic compounds. This whole saliva stands for secretions from major (submandibular, sublingual and parotid) and minor salivary glands, along with crevicular fluid, bacteria and cellular debris. The secretion of saliva is regulated under the autonomic nervous system via signal transduction systems that couple receptor stimulation to ion transport and protein secretory mechanisms (Dodds, Johnson, & Yeh, 2005). SDS-PAGE is a unidimensional method used to analyze the molecular weights and charges of proteins by separating the proteins into bands, thereby making them visible. The most common use of gel electrophoresis is the qualitative analysis of the complex mixtures of proteins. Schwartz et al. proposed polyacrylamide gel electrophoresis (SDS-PAGE) as an adequate method to evaluate protein content in the human saliva. In this work it was aimed to study the saliva protein composition from different subjects by high resolution liquid chromatography mass spectrometry (HR-LC-MS/MS) on different days, throughout the menstrual cycle and the influence of ovulation.
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Professor and Head, Department of Animal Science,
Bharathidasan University |
Gel-based experiment |
2025-01-25 |
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| 7 |
IPD4671 |
Human salivary proteome to map biomarker for ectopic pregnancy |
Dr Govindaraj Archunan |
Explore the profile of salivary proteins with an attention during ectopic pregnancy adopting gel based protein mass spectrometry
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Professor and Head, Department of Animal Science,
Bharathidasan University |
Gel-based experiment |
2025-01-25 |
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| 8 |
IPD5805 |
Salivary proteins in buffalo with special emphasis to estrous cycle |
Dr Govindaraju Archunan |
Saliva is considered as the best source for biomarker-discovery studies, since it is a non-invasive method when compared to other body sources. Usually, buffalo cannot express their estrus signs precisely. Hence, there is a need for concise methods to detect the time of estrus to ensure the success in artificial...
Saliva is considered as the best source for biomarker-discovery studies, since it is a non-invasive method when compared to other body sources. Usually, buffalo cannot express their estrus signs precisely. Hence, there is a need for concise methods to detect the time of estrus to ensure the success in artificial insemination. Therefore, we have established a reference proteome map on buffalo whole saliva during estrous cycle in order to document the estrus-specific proteins using SDS-PAGE and mass spectrometry. The present findings conclude that the proteomic approach adopted to identify the proteins from buffalo saliva around estrous cycle may provide a new tool for screening the estrus phase.
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Professor and Head, Department of Animal Science,
Bharathidasan University |
Shotgun proteomics |
2025-01-25 |
25114174
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| 9 |
IPD8273 |
Analysis of low molecular weight protein profile in whole saliva with special reference to ovulation |
Dr Archunan Govindaraju |
Whole saliva is contains major, minor and gingival crevicular fluid/secretions and it plays vital role in maintaining the oral health, three pair of major salivary glands consist of parotid, submandibular and sub-lingual glands. Saliva is a good biological fluid to explore health and disease status of human....
Whole saliva is contains major, minor and gingival crevicular fluid/secretions and it plays vital role in maintaining the oral health, three pair of major salivary glands consist of parotid, submandibular and sub-lingual glands. Saliva is a good biological fluid to explore health and disease status of human. Further, the saliva secrets from major salivary glands and have various biomolecules such as, proteins, enzymes and ions originated from serum and saliva is easily accessible through a safe and noninvasive method. In contrast, the concentrations of biomolecules in saliva are generally one tenth to one thousand of the levels in blood. Ovulation is an exclusive process through the mature (pre-ovulatory) ovarian follicles reacts to the surge of luteinizing hormone (LH) and burst to discharge fertilizable oocytes. The LH surge initiates a flow of proteolytic actions with the aim of control ovulation. Presently, many salivary proteins are have been showed as a biomarker such as Sjogren’s syndrome, Lung cancer, oral and Systemic diseases, HIV-seropositive patients, dental pellicle development, and hyperglycemia. However, there is no prominent marker protein was reported for monitoring ovulation process in human saliva. It is important prediction of fertile period in women for various applications like assisted reproduction and invitro fertilization.
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Professor and Head, Department of Animal Science,
Bharathidasan University |
Shotgun proteomics |
2025-01-25 |
27802913
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| 10 |
IPD7165 |
Exploration of buffalo CVM Staphylococcus pasteuri proteins during estrus by LC-MSMS |
Dr Govindaraju Archunan |
The exploration of the secretary proteins of Staphylococcus pasteuri during estrus using the SDS page followed by LC-MS/MS.
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Professor and Head, Department of Animal Science,
Bharathidasan University |
Gel-based experiment |
2025-01-25 |
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