Indian Biological Images Archive

Project Accession:	IBIAP_1000000028
Title:	Histopathological Evaluation of Virus-Induced Pulmonary Changes in HCoV-229E–Infected BALB/c Mice
Representative Image:
Description:	The dataset includes lung tissue sections from BALB/c mice stained with Haematoxylin and Eosin (H&E) and Masson's Trichrome (MT) to assess microscopic changes following HCoV-229E infection. H&E staining was used to evaluate cellular infiltration, tissue architecture, and inflammation, while MT staining was utilized to examine collagen deposition and fibrosis-related changes, which are commonly observed in severe coronavirus infections such as SARS-CoV-2.As BALB/c mice are not a naturally permissive model for HCoV-229E replication—due to the absence of the human aminopeptidase-N (hAPN) receptor—the histopathological analysis primarily aims to compare infected versus uninfected lung tissues to determine whether any inflammatory, thrombotic, or fibrotic changes are triggered despite limited viral replication. This dataset provides qualitative insight into the immunogenic effects of 229E exposure in a non-transgenic mouse model and supports its potential use for preliminary immunogenicity assessments relevant to vaccine development, rather than for studying full viral pathogenesis.
Publications:	N/A
Associated Codes (URL only):	N/A
Funding agency:
Grant Number:
Ethics Statement:	N/A
Any Other Information :	N/A
Additional File:	N/A
Acknowledgments:	The current research is supported by the Translational Research Program Grant, BT/PR30159/MED/15/188/2018, THSTI Core (Intramural P189, P417, P542, P548 & T001), The ICMR-National One Health Mission (ICMR/DHR/NOHM/SN ID-28/2025/04/14) and (ICMR/DHR/NOHM/SN ID-29/2025/04/15). We also extend our sincere thanks to the Small Animal Facility and the Immunology Core at THSTI for their valuable technical assistance and essential support throughout the experimental work.

Sr.No	First name	Last name	Email	Organization	Designation
1	Navya	Chauhan	Navya.chauhan@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
2	Nisha	Beniwal	Beniwalnisha7@gmail.com	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
3	Sushma	Mithani	sushma@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
4	Banwari	Lal	Banwarillaw6633@gmail.com	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
5	Sudipta	Sonar	Sudipta@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
6	Kamini	Jakhar	Jakharkamini@gmail.com	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
7	Chandan	Verma	Chandanverma@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
8	Sakshi	Nautiyal	Nautiyalsakshi@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
9	Rimpy		Rimpy@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
10	Vikas	Phagna	Phagna069@gmail.com	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
11	Satendra	Kumar	Satendra@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
12	Anchal	Raj	Anchalraj@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Research Scholar
13	Shailendra	Mani	Shailendramani@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Principal Investigator
14	Sankar	Bhattacharyya	Sankar@thsti.res.in	Translational Health Science and Technology Institute (THSTI), Faridabad, India	Co-Principal Investigator

Study Accession:	HISTOS_1000000033
Title:	Investigating the Immunogenicity of Human Coronavirus 229E in BALB/c Mice via Multiple Routes of Infection
Imaging Type:	Histopathology (HISTO)
Imaging Sub-type:	Diagnostic Pathology
Summary:	Human coronavirus 229E (HCoV-229E) is an alphacoronavirus that typically causes mild respiratory illness but can be severe in vulnerable individuals. Due to the absence of the human aminopeptidase-N (hAPN) receptor, BALB/c mice are not naturally permissive to productive 229E replication. This study evaluates the immunogenicity of HCoV-229E in BALB/c mice and examines how different routes of infection shape antibody responses. Mice were inoculated intranasally, intraperitoneally, or intramuscularly, with mock animals as controls. Antibody responses were quantified using Luminex IgG assays and microneutralization tests, while lung tissues were assessed by qPCR and histopathology using H&E and Masson’s Trichrome staining. All infection routes elicited measurable humoral responses, with the intraperitoneal route inducing the highest IgG and neutralizing antibody titers. These findings support the utility of BALB/c mice for preliminary immunogenicity studies and provide insight into optimising antigen delivery routes for vaccines and antiviral strategies.
Keywords:	Human coronavirus 229E (HCoV-229E); BALB/c mice; Immunogenicity; Infection route; Antibody response; Neutralizing antibodies; Viral load; qPCR; Histopathology; Cellular infiltration; Masson's Trichrome; Hematoxylin and Eosin; Vaccine development
Additional / Any Other Information:	N/A
Release Date:	Dec. 23, 2025
Access Licence Type:	Open Access

Table 1. The sample types registered under this study are as follows:

Sample Type ID	Organism	Taxon ID	Biological Entity	Laterality	Source Tissue	Source Cell/Cell-line	Cell Organelle
HISTOSMT_10000000065	Mus musculus	10090	Lung	Not Available	Lung tissue	N/A	N/A

The total number of samples registered under this study is: 160

Table 3. The experiment types registered under this study are as follows:

Experiment Type ID	Instrument Name	Instrument Type	Manufacturer	Model
HISTOET_10000000031	Microscope	Inverted microscope	Nikon	Nikon Eclipse Ti
HISTOET_10000000032	Microscope	Inverted microscope	Nikon	Nikon Eclipse Ti

Experimental Design Summary (HISTOET_10000000031)
Histopathology images were acquired using a Nikon Eclipse Ti Inverted Microscope fitted with a digital camera at 20× objective × 10× eyepiece magnification, with each image captured as a 3 × 3 stitched field. Lung tissues were harvested and fixed immediately in 4% buffered formalin for 48 hours, followed by dehydration in graded alcohols, xylene clearing, and paraffin embedding. 3 µm sections were prepared on glass slides, deparaffinized, and stained using standard H&E and Masson’s Trichrome protocols. Whole lung sections, containing epithelium, alveoli, bronchioles, vessels,etc were examined to assess inflammation, thrombosis, and collagen deposition.

Experimental Design Summary (HISTOET_10000000031)

Histopathology images were acquired using a Nikon Eclipse Ti Inverted Microscope fitted with a digital camera at 20× objective × 10× eyepiece magnification, with each image captured as a 3 × 3 stitched field. Lung tissues were harvested and fixed immediately in 4% buffered formalin for 48 hours, followed by dehydration in graded alcohols, xylene clearing, and paraffin embedding. 3 µm sections were prepared on glass slides, deparaffinized, and stained using standard H&E and Masson’s Trichrome protocols. Whole lung sections, containing epithelium, alveoli, bronchioles, vessels,etc were examined to assess inflammation, thrombosis, and collagen deposition.

Acquired Images Annotation Description (HISTOET_10000000031)
Images show H&E- and MT-stained whole-lung sections captured at 20× magnification using a Nikon Eclipse Ti microscope. Annotated regions highlight cellular infiltration, mild thrombosis, and collagen deposition. All major lung structures-epithelium, alveoli, bronchioles, vessels,etc—are visible for assessment of histopathological changes.

Experimental Design Summary (HISTOET_10000000032)
Histopathological images were acquired using a Nikon Eclipse Ti Inverted Microscope fitted with a digital camera at 20× objective × 10× eyepiece magnification, with each image captured as a 3 × 3 stitched field. Lung tissues were harvested and fixed immediately in 4% buffered formalin for 48 hours, followed by dehydration in graded alcohols, xylene clearing, and paraffin embedding. 3 µm sections were prepared on glass slides, deparaffinized, and stained using standard H&E and Masson’s Trichrome protocols. Whole lung sections, containing epithelium, alveoli, bronchioles, vessels,etc were examined to assess inflammation, thrombosis, and collagen deposition.

Experimental Design Summary (HISTOET_10000000032)

Histopathological images were acquired using a Nikon Eclipse Ti Inverted Microscope fitted with a digital camera at 20× objective × 10× eyepiece magnification, with each image captured as a 3 × 3 stitched field. Lung tissues were harvested and fixed immediately in 4% buffered formalin for 48 hours, followed by dehydration in graded alcohols, xylene clearing, and paraffin embedding. 3 µm sections were prepared on glass slides, deparaffinized, and stained using standard H&E and Masson’s Trichrome protocols. Whole lung sections, containing epithelium, alveoli, bronchioles, vessels,etc were examined to assess inflammation, thrombosis, and collagen deposition.

Acquired Images Annotation Description (HISTOET_10000000032)
Images show H&E- and MT-stained whole-lung sections captured at 20× magnification using a Nikon Eclipse Ti microscope. Annotated regions highlight cellular infiltration, mild thrombosis, and collagen deposition. All major lung structures—epithelium, alveoli, bronchioles, vessels,etc—are visible for assessment of histopathological changes.

The total number of experiments registered under this study is: 160

The total number of images registered under this study is: 160

IBIA: Indian Biological Images Archive

Address

Quick Links

IBIA: Indian Biological Images Archive

Project

Authors/Collaborators

Study

Sample(s)

Experiment(s)

Image Files

Address

Quick Links