9V26 image
Deposition Date 2025-05-19
Release Date 2025-10-08
Last Version Date 2025-10-08
Entry Detail
PDB ID:
9V26
Keywords:
RIBOSOME
Title:
Cryo- EM structure of 75S ribosome with A/P- & P/E- tRNAs from Entamoeba histolytica bound to antibiotic paromomycin
Biological Source:
Source Organism(s):
Entamoeba histolytica HM-1:IMSS (Taxon ID: 294381)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
3.10 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
9V26 validation report
Macromolecular Entities
Polymer Type:polyribonucleotide
Molecule:25S rRNA
Chain IDs:A (auth: lA)
Chain Length:213
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
Polymer Type:polyribonucleotide
Molecule:5.8S rRNA
Chain IDs:B (auth: lB)
Chain Length:155
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
Polymer Type:polyribonucleotide
Molecule:5S rRNA
Chain IDs:C (auth: lC)
Chain Length:149
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL8
Chain IDs:D (auth: lD)
Chain Length:64
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:O15574 Pfam ID:PF00181, PF03947
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L3, put
Gene (Uniprot):EHI_005890, EHI_055670, EHI_126240
Chain IDs:E (auth: lE)
Chain Length:402
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LW09 Pfam ID:PF00297
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L4, put
Gene (Uniprot):EHI_064710
Chain IDs:F (auth: lF)
Chain Length:150
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:B1N384 Pfam ID:PF00573, PF14374
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L5, put
Gene (Uniprot):EHI_006860, EHI_068660
Chain IDs:G (auth: lG)
Chain Length:134
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LU56 Pfam ID:PF17144, PF14204
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL6
Chain IDs:H (auth: lH)
Chain Length:137
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:O15595 Pfam ID:PF01159
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L7, put
Gene (Uniprot):EHI_192110
Chain IDs:I (auth: lI)
Chain Length:230
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M751 Pfam ID:PF00327
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L7a
Gene (Uniprot):EHI_103310
Chain IDs:J (auth: lJ)
Chain Length:108
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LVH6 Pfam ID:PF01248
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L9, put
Gene (Uniprot):EHI_126140
Chain IDs:K (auth: lK)
Chain Length:104
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LVZ9 Pfam ID:PF00347
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L10, putati
Gene (Uniprot):EHI_044810, EHI_098780
Chain IDs:L (auth: lL)
Chain Length:77
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LTA0 Pfam ID:PF00252
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L11, pu
Gene (Uniprot):EHI_124300, EHI_199970
Chain IDs:M (auth: lM)
Chain Length:174
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LZG9 Pfam ID:PF00281, PF00673
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L13, pu
Gene (Uniprot):EHI_181560
Chain IDs:N (auth: lN)
Chain Length:291
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M8R8 Pfam ID:PF01294
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L13, pu
Gene (Uniprot):EHI_017700, EHI_099730
Chain IDs:O (auth: lO)
Chain Length:205
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M3F3 Pfam ID:PF00572
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L14, pu
Gene (Uniprot):EHI_185580
Chain IDs:P (auth: lP)
Chain Length:56
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M8C9 Pfam ID:PF00467, PF01929
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L15
Gene (Uniprot):EHI_020300, EHI_040800
Chain IDs:Q (auth: lQ)
Chain Length:98
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M7U2 Pfam ID:PF00827
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L17, pu
Gene (Uniprot):EHI_127330
Chain IDs:R (auth: lR)
Chain Length:179
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LX05 Pfam ID:PF00237
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L18, pu
Gene (Uniprot):EHI_079190, EHI_098470, EHI_187740
Chain IDs:S (auth: lS)
Chain Length:168
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LXC8 Pfam ID:PF17135
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L18a
Gene (Uniprot):EHI_035600, EHI_090020, EHI_131940, EHI_163670
Chain IDs:T (auth: lT)
Chain Length:173
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LXZ0 Pfam ID:PF01775
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L19
Gene (Uniprot):EHI_096850
Chain IDs:U (auth: lU)
Chain Length:198
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LWJ4 Pfam ID:PF01280, PF25476
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L21, pu
Gene (Uniprot):EHI_161180, EHI_182920
Chain IDs:V (auth: lV)
Chain Length:166
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LV69 Pfam ID:PF01157
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):EHI_141940
Chain IDs:W (auth: lW)
Chain Length:137
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LW40 Pfam ID:PF01776
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L23, pu
Gene (Uniprot):EHI_051710, EHI_193330, EHI_196920
Chain IDs:X (auth: lX)
Chain Length:140
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LVB0 Pfam ID:PF00238
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L23A, putat
Gene (Uniprot):EHI_182850
Chain IDs:Y (auth: lY)
Chain Length:121
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LV64 Pfam ID:PF00276
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L24, pu
Gene (Uniprot):EHI_030760, EHI_153080
Chain IDs:Z (auth: lZ)
Chain Length:163
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LSA0 Pfam ID:PF01246
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L26, pu
Gene (Uniprot):EHI_152570
Chain IDs:AA (auth: la)
Chain Length:213
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LSU3 Pfam ID:PF16906
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L27, pu
Gene (Uniprot):EHI_183480
Chain IDs:BA (auth: lb)
Chain Length:155
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M727 Pfam ID:PF00467, PF01777
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):rpl27a-1
Chain IDs:CA (auth: lc)
Chain Length:149
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:Q9BI06 Pfam ID:PF00828
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L29
Gene (Uniprot):EHI_023220
Chain IDs:DA (auth: ld)
Chain Length:64
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LUN3 Pfam ID:PF01779
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L30, pu
Gene (Uniprot):EHI_192800
Chain IDs:EA (auth: le)
Chain Length:402
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M919 Pfam ID:PF01248
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L31, pu
Gene (Uniprot):EHI_068200, EHI_140370
Chain IDs:FA (auth: lf)
Chain Length:150
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LU13 Pfam ID:PF01198
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L32, pu
Gene (Uniprot):EHI_044770
Chain IDs:GA (auth: lg)
Chain Length:134
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LT96 Pfam ID:PF01655
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L34, pu
Gene (Uniprot):EHI_167050
Chain IDs:HA (auth: lh)
Chain Length:137
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LYA7 Pfam ID:PF01199
Polymer Type:polypeptide(L)
Molecule:uL29
Chain IDs:IA (auth: li)
Chain Length:230
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L35a, p
Gene (Uniprot):EHI_014110, EHI_015310, EHI_025590, EHI_049000, EHI_114920
Chain IDs:JA (auth: lj)
Chain Length:108
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:B1N4V3 Pfam ID:PF01247
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L36, pu
Gene (Uniprot):EHI_124330, EHI_184480, EHI_199940
Chain IDs:KA (auth: lk)
Chain Length:104
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LZH1 Pfam ID:PF01158
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L37-A,
Gene (Uniprot):EHI_015320, EHI_048990, EHI_156690, EHI_158270
Chain IDs:LA (auth: ll)
Chain Length:77
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M0X0 Pfam ID:PF01907
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L37a, p
Gene (Uniprot):EHI_008650
Chain IDs:MA (auth: lm)
Chain Length:174
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:A0A175JXH6 Pfam ID:PF01780
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L38 put
Gene (Uniprot):CL6EHI_023840, CL6EHI_099790
Chain IDs:NA (auth: ln)
Chain Length:291
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:A0A175JTS8 Pfam ID:PF01781
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L39, putati
Gene (Uniprot):EHI_044830, EHI_143430
Chain IDs:OA (auth: lo)
Chain Length:205
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LTA2 Pfam ID:PF00832
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L40, pu
Gene (Uniprot):EHI_092470, EHI_119590, EHI_170340
Chain IDs:PA (auth: lp)
Chain Length:56
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LV09 Pfam ID:PF01020
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L44, pu
Gene (Uniprot):EHI_092120, EHI_153070, EHI_171300
Chain IDs:QA (auth: lq)
Chain Length:98
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LS99 Pfam ID:PF00935
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S25
Gene (Uniprot):EHI_074800, EHI_189150
Chain IDs:RA (auth: sA)
Chain Length:1947
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M9F4 Pfam ID:PF03297
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S26
Gene (Uniprot):EHI_158550
Chain IDs:SA (auth: sB)
Chain Length:144
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M7G9 Pfam ID:PF01283
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS27
Chain IDs:TA (auth: sC)
Chain Length:255
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:P38654 Pfam ID:PF01667
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S28, pu
Gene (Uniprot):EHI_038860
Chain IDs:UA (auth: sD)
Chain Length:69
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LZQ8 Pfam ID:PF01200
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein S29, putati
Gene (Uniprot):EHI_030070, EHI_126250
Chain IDs:VA (auth: sE)
Chain Length:56
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LW10 Pfam ID:PF00253
Polymer Type:polyribonucleotide
Molecule:A/P-tRNA
Chain IDs:WA (auth: sI)
Chain Length:201
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
Polymer Type:polyribonucleotide
Molecule:P/E-tRNA
Chain IDs:XA (auth: sJ)
Chain Length:77
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
Polymer Type:polyribonucleotide
Molecule:mRNA
Chain IDs:YA (auth: sK)
Chain Length:10
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
Polymer Type:polyribonucleotide
Molecule:17S rRNA
Chain IDs:ZA (auth: sa)
Chain Length:1947
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):EHI_020280
Chain IDs:AB (auth: sc)
Chain Length:255
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M7U0 Pfam ID:PF00333, PF03719
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S3
Gene (Uniprot):EHI_146340, EHI_148320
Chain IDs:BB (auth: sd)
Chain Length:69
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LT02 Pfam ID:PF07650, PF00189
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):EHI_065270, EHI_072040
Chain IDs:CB (auth: se)
Chain Length:56
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M609 Pfam ID:PF01015
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S4, put
Gene (Uniprot):EHI_003940, EHI_008210, EHI_098840
Chain IDs:DB (auth: sf)
Chain Length:326
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LYF4 Pfam ID:PF08071, PF00900, PF16121
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS5
Chain IDs:EB (auth: sg)
Chain Length:206
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:O15587 Pfam ID:PF00177
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S6
Gene (Uniprot):EHI_013890
Chain IDs:FB (auth: sh)
Chain Length:266
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LXN2 Pfam ID:PF01092
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S7
Gene (Uniprot):EHI_067530
Chain IDs:GB (auth: si)
Chain Length:201
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M9F9 Pfam ID:PF01251
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S8
Gene (Uniprot):EHI_086510
Chain IDs:HB (auth: sj)
Chain Length:77
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M8Y8 Pfam ID:PF01201
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):2.t00127, 6.t00069, 11.t00056
Chain IDs:IB (auth: sk)
Chain Length:10
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:O15612 Pfam ID:PF00163, PF01479
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S10, pu
Gene (Uniprot):EHI_197030
Chain IDs:JB (auth: sl)
Chain Length:127
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LVC0 Pfam ID:PF03501
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S11, pu
Gene (Uniprot):EHI_124850
Chain IDs:KB (auth: sm)
Chain Length:156
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M979 Pfam ID:PF16205, PF00366
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S13, pu
Gene (Uniprot):EHI_035460
Chain IDs:LB (auth: so)
Chain Length:151
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:A0A175JJM9 Pfam ID:PF08069, PF00312
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein S14, putati
Gene (Uniprot):EHI_012360, EHI_074090
Chain IDs:MB (auth: sp)
Chain Length:146
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:B1N2I3 Pfam ID:PF00411
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S15, pu
Gene (Uniprot):EHI_111980
Chain IDs:NB (auth: sq)
Chain Length:144
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M7H3 Pfam ID:PF00203
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S15a, p
Gene (Uniprot):EHI_073600, EHI_105140, EHI_167270
Chain IDs:OB (auth: sr)
Chain Length:130
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LXM0 Pfam ID:PF00410
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S16, pu
Gene (Uniprot):EHI_160990
Chain IDs:PB (auth: ss)
Chain Length:158
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M2R7 Pfam ID:PF00380
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S17, pu
Gene (Uniprot):EHI_135060
Chain IDs:QB (auth: st)
Chain Length:117
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M0Z2 Pfam ID:PF00833
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS18
Chain IDs:RB (auth: su)
Chain Length:155
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:P48151 Pfam ID:PF00416
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS19
Chain IDs:SB (auth: sv)
Chain Length:155
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:O15631 Pfam ID:PF01090
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S20, pu
Gene (Uniprot):EHI_026410
Chain IDs:TB (auth: sw)
Chain Length:118
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M5V7 Pfam ID:PF00338
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S21
Gene (Uniprot):EHI_126870
Chain IDs:UB (auth: sx)
Chain Length:86
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4LWW3 Pfam ID:PF01249
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S23, pu
Gene (Uniprot):EHI_020310, EHI_040830, EHI_081320, EHI_108500
Chain IDs:VB (auth: sy)
Chain Length:141
Number of Molecules:1
Biological Source:Entamoeba histolytica HM-1:IMSS
UniProt ID:C4M5B7 Pfam ID:PF00164
Ligand Molecules
PAR
Primary Citation
Cryo-EM structure of ribosome from pathogenic protozoa Entamoeba histolytica reveals unique features of its architecture.
Nat Commun 16 7758 7758 (2025)
PMID: 40835601 DOI: 10.1038/s41467-025-62767-x

Abstact

Entamoeba histolytica, an anaerobic protozoan parasite, is the causative agent of amoebiasis, bloody diarrhea, and liver abscesses in humans. Amoebiasis is more predominant in tropical areas with poor sanitation conditions, and it remains the fourth leading cause of death due to a protozoan infection. E. histolytica life cycle spans between an infective 'cyst stage' and an active disease-causing 'trophozoite stage'. We have isolated ribosomes from the trophozoite stage of E. histolytica. Here, we report single particle cryo-EM structures of the 53S ribosome large subunit (LSU), and 75S associated ribosomes, with P-tRNA, A/P and P/E tRNAs, and with paromomycin antibiotic, at 2.8 Å to 3.4 Å resolution. The E. histolytica possesses a reduced ribosome with a conserved core, and the periphery evolved with species-specific unique features. The most notable features are the presence of the rRNA triple helix near the peptide exit tunnel, the expansion segment H88ES102 near the exit site on LSU, and unique insertions in r-proteins. Furthermore, the 75S ribosome paromomycin complex structure provides the atomic details of its interactions. These structures provide insights into the evolutionary adaptation of the E. histolytica translational machinery and may be explored further for amoebicidal therapeutic intervention.

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Protein

Chemical

Disease

Primary Citation of related structures
9V26
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