ISDA: 9U53

Deposition Date 2025-03-20

Release Date 2025-09-24

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9U53

Keywords:

OXIDOREDUCTASE

Title:

Crystal structure of the CYP154C2 Q230A from Streptomyces avermitilis

Biological Source:

Source Organism(s):

Streptomyces avermitilis (Taxon ID: 33903)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.97 Å

R-Value Free:

0.23

R-Value Work:

0.17

R-Value Observed:

0.18

Space Group:

P 21 21 21

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Cytochrome P450
Gene (Uniprot):SAV14893_032670, SAV31267_054670
Mutagens:Q230A
Chain IDs:A
Chain Length:420
Number of Molecules:1
Biological Source:Streptomyces avermitilis

UniProt ID:A0A4D4LTT1 Pfam ID:PF00067

Ligand Molecules

EDO

HEM

PEG

Primary Citation

Conformational gating in CYP154C2: Gln230-mediated substrate recognition and catalytic switching revealed by structural dynamics.

Yang, J, Huang, J, He, X, Fushinobu, S, Xu, L.H.Show

Biosci.Biotechnol.Biochem. 89 1144 1153 (2025)

PMID: 40408304 DOI: 10.1093/bbb/zbaf076

Primary Citation of related structures

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