ISDA: 9DVT

Deposition Date 2024-10-08

Release Date 2025-10-15

Last Version Date 2026-03-04

Entry Detail

PDB ID:

9DVT

Keywords:

DNA BINDING PROTEIN/DNA

Title:

Cryo-EM structure of RpaA bound to PkaiBC DNA and the CTD of the alpha subunit of RNAP from the cyanobacterium Synechococcus elongatus

Biological Source:

Source Organism(s):

Synechococcus elongatus (Taxon ID: 32046)
Synechococcus elongatus PCC 7942 = FACHB-805 (Taxon ID: 1140)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.74 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polydeoxyribonucleotide
Molecule:Non-template DNA
Chain IDs:A (auth: 1)
Chain Length:105
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Template DNA
Chain IDs:B (auth: 2)
Chain Length:105
Number of Molecules:1
Biological Source:synthetic construct

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:C (auth: A)
Chain Length:309
Number of Molecules:1
Biological Source:Synechococcus elongatus

UniProt ID:Q31L30 Pfam ID:PF01193, PF01000, PF03118

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:RNA polymerase sigma factor S
Gene (Uniprot):sigA1
Chain IDs:F (auth: G)
Chain Length:399
Number of Molecules:1
Biological Source:Synechococcus elongatus PCC 7942 = FACHB-805

UniProt ID:P38023 Pfam ID:PF00140, PF04542, PF04539, PF04545

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-binding dual master trans
Gene (Uniprot):rpaA
Chain IDs:D (auth: R), E (auth: S)
Chain Length:249
Number of Molecules:2
Biological Source:Synechococcus elongatus PCC 7942 = FACHB-805

UniProt ID:Q31S42 Pfam ID:PF00072, PF00486

Modified Residue