ISDA: 6ADQ

Deposition Date 2018-08-01

Release Date 2018-11-14

Last Version Date 2025-09-17

Entry Detail

PDB ID:

6ADQ

Keywords:

ELECTRON TRANSPORT

Title:

Respiratory Complex CIII2CIV2SOD2 from Mycobacterium smegmatis

Biological Source:

Source Organism(s):

Mycobacterium smegmatis MC2 51 (Taxon ID: 1445611)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.50 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Rieske iron-sulfur protein Qc
Gene (Uniprot):qcrA
Chain IDs:L (auth: A), X (auth: M)
Chain Length:421
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:I7GD61 Pfam ID:PF19297, PF00355

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ubiquinol-cytochrome c reduct
Gene (Uniprot):MSMEG_4263
Chain IDs:J (auth: B), V (auth: N)
Chain Length:546
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0R052 Pfam ID:PF13631

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Cytochrome bc1 complex cytoch
Gene (Uniprot):qcrC
Chain IDs:K (auth: C), W (auth: O)
Chain Length:294
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:I7FPH1 Pfam ID:PF00034, PF13442

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Prokaryotic respiratory super
Chain IDs:G (auth: D), S (auth: P)
Chain Length:100
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0QVH4 Pfam ID:PF10939

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):MSMEG_4268
Chain IDs:A (auth: E), M (auth: Q)
Chain Length:341
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0R057 Pfam ID:PF00116

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Chain IDs:B (auth: F), N (auth: R)
Chain Length:575
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:6ADQ

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):MSMEG_4260
Chain IDs:C (auth: G), O (auth: S)
Chain Length:203
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0R049 Pfam ID:PF00510

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase polypept
Gene (Uniprot):MSMEG_4267
Chain IDs:D (auth: H), P (auth: T)
Chain Length:139
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0R056 Pfam ID:PF12270

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):MSMEG_4693
Chain IDs:E (auth: I), Q (auth: U)
Chain Length:79
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0R1B6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Uncharacterized protein MSMEG
Gene (Uniprot):MSMEG_4692, MSMEI_4575
Chain IDs:F (auth: J), R (auth: V)
Chain Length:157
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0R1B5 Pfam ID:PF17174

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:LpqE protein
Gene (Uniprot):MSMEG_6078
Chain IDs:I (auth: K), U (auth: W)
Chain Length:186
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0R562 Pfam ID:PF04314

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Superoxide dismutase [Cu-Zn]
Gene (Uniprot):sodC
Chain IDs:H (auth: Y), T (auth: Z)
Chain Length:236
Number of Molecules:2
Biological Source:Mycobacterium smegmatis MC2 51

UniProt ID:A0QQQ1 Pfam ID:PF00080

Ligand Molecules

9XX

9Y0

9YF

CDL

FES

HEA

HEC

HEM

MQ9

PLM

Primary Citation

An electron transfer path connects subunits of a mycobacterial respiratory supercomplex.

Gong, H, Li, J, Xu, A, Tang, Y, Ji, W, Gao, R, Wang, S, Yu, L, Tian, C, Li, J, Yen, H.Y, Man Lam, S, Shui, G, Yang, X, Sun, Y, Li, X, Jia, M, Yang, C, Jiang, B, Lou, Z, Robinson, C.V, Wong, L.L, Guddat, L.W, Sun, F, Wang, Q, Rao, Z.Show

Science 362 ? ? (2018)

PMID: 30361386 DOI: 10.1126/science.aat8923

Abstact

We report a 3.5-angstrom-resolution cryo-electron microscopy structure of a respiratory supercomplex isolated from Mycobacterium smegmatis. It comprises a complex III dimer flanked on either side by individual complex IV subunits. Complex III and IV associate so that electrons can be transferred from quinol in complex III to the oxygen reduction center in complex IV by way of a bridging cytochrome subunit. We observed a superoxide dismutase-like subunit at the periplasmic face, which may be responsible for detoxification of superoxide formed by complex III. The structure reveals features of an established drug target and provides a foundation for the development of treatments for human tuberculosis.

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Primary Citation of related structures

Abstact

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