ISDA: 5ZTE

Deposition Date 2018-05-03

Release Date 2018-10-10

Last Version Date 2023-11-22

Entry Detail

PDB ID:

5ZTE

Keywords:

OXIDOREDUCTASE

Title:

Crystal structure of PrxA C119S mutant from Arabidopsis thaliana

Biological Source:

Source Organism(s):

Arabidopsis thaliana (Taxon ID: 3702)

Expression System(s):

Escherichia coli bl21

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.60 Å

R-Value Free:

0.24

R-Value Work:

0.19

R-Value Observed:

0.19

Space Group:

C 1 2 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:2-Cys peroxiredoxin BAS1, chl
Gene (Uniprot):BAS1
Mutagens:C119S
Chain IDs:A, B, C, D, E, F, G, H, I, J
Chain Length:203
Number of Molecules:10
Biological Source:Arabidopsis thaliana

UniProt ID:Q96291 Pfam ID:PF00578, PF10417 EC:1.11.1.15

Primary Citation

Crystal structure of Arabidopsis thaliana peroxiredoxin A C119S mutant.

Yang, Y, Cai, W, Wang, J, Pan, W, Liu, L, Wang, M, Zhang, M.Show

Acta Crystallogr F Struct Biol Commun 74 625 631 (2018)

PMID: 30279313 DOI: 10.1107/S2053230X18010920

Abstact

Peroxiredoxins (Prxs), a large family of antioxidant enzymes, are abundant in all living organisms. Peroxiredoxin A (PrxA) from Arabidopsis thaliana belongs to the typical 2-Cys Prx family and is localized in the chloroplast. This article reports the crystal structure of a PrxA C119S mutant refined to 2.6 Å resolution. The protein exists as a decamer both in the crystal structure and in solution. The structure is in the reduced state suitable for the approach of peroxide, though conformational changes are needed for the resolving process.

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Primary Citation of related structures

Abstact

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