ISDA: 4BTS

Deposition Date 2013-06-19

Release Date 2013-07-17

Last Version Date 2023-12-20

Entry Detail

PDB ID:

4BTS

Keywords:

RIBOSOME

Title:

THE CRYSTAL STRUCTURE OF THE EUKARYOTIC 40S RIBOSOMAL SUBUNIT IN COMPLEX WITH EIF1 AND EIF1A

Biological Source:

Source Organism(s):

Tetrahymena thermophila SB210 (Taxon ID: 312017)
TETRAHYMENA THERMOPHILA (Taxon ID: 5911)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

3.70 Å

R-Value Free:

0.22

R-Value Work:

0.20

R-Value Observed:

0.20

Space Group:

P 1 21 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:TRANSLATION INITIATION FACTOR
Gene (Uniprot):TTHERM_00497990
Chain IDs:A (auth: A0), KA (auth: B0), UB (auth: C0), ED (auth: D0)
Chain Length:211
Number of Molecules:4
Biological Source:Tetrahymena thermophila SB210

UniProt ID:I7MK25 Pfam ID:PF01176

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS28E
Chain IDs:B (auth: A1), LA (auth: B1), VB (auth: C1), FD (auth: D1)
Chain Length:68
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU6 Pfam ID:PF01200

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN S8
Chain IDs:C (auth: A2), MA (auth: B2), WB (auth: C2), GD (auth: D2)
Chain Length:208
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS6 Pfam ID:PF01201

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS7E
Chain IDs:D (auth: A3), NA (auth: B3), XB (auth: C3), HD (auth: D3)
Chain Length:197
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS5 Pfam ID:PF01251

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN S3A
Chain IDs:E (auth: A4), OA (auth: B4), YB (auth: C4), ID (auth: D4)
Chain Length:265
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBR8 Pfam ID:PF01015

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS26E
Chain IDs:F (auth: A5), PA (auth: B5), ZB (auth: C5), JD (auth: D5)
Chain Length:119
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU4 Pfam ID:PF01283

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN S27
Chain IDs:G (auth: A6), QA (auth: B6), AC (auth: C6), KD (auth: D6)
Chain Length:81
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU5 Pfam ID:PF01667

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS10E
Chain IDs:H (auth: A7), RA (auth: B7), BC (auth: C7), LD (auth: D7)
Chain Length:162
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS8 Pfam ID:PF03501

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS25E
Chain IDs:I (auth: A8), SA (auth: B8), CC (auth: C8), MD (auth: D8)
Chain Length:143
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU3 Pfam ID:PF03297

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS31E
Chain IDs:J (auth: A9), TA (auth: B9), DC (auth: C9), ND (auth: D9)
Chain Length:189
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU9 Pfam ID:PF01599

Polymer Type:polyribonucleotide
Molecule:18S ribosomal RNA
Chain IDs:K (auth: AA), UA (auth: BA), EC (auth: CA), OD (auth: DA)
Chain Length:1753
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN SA
Chain IDs:L (auth: AB), VA (auth: BB), FC (auth: CB), PD (auth: DB)
Chain Length:241
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBR9 Pfam ID:PF00318

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS3E
Chain IDs:M (auth: AC), WA (auth: BC), GC (auth: CC), QD (auth: DC)
Chain Length:243
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS1 Pfam ID:PF07650, PF00189

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS9E
Chain IDs:N (auth: AD), XA (auth: BD), HC (auth: CD), RD (auth: DD)
Chain Length:181
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS7 Pfam ID:PF00163, PF01479

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS2E
Chain IDs:O (auth: AE), YA (auth: BE), IC (auth: CE), SD (auth: DE)
Chain Length:296
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS0 Pfam ID:PF00333, PF03719

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:EIF1
Chain IDs:P (auth: AF), ZA (auth: BF), JC (auth: CF), TD (auth: DF)
Chain Length:101
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBV1 Pfam ID:PF01253

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS5E
Chain IDs:Q (auth: AG), AB (auth: BG), KC (auth: CG), UD (auth: DG)
Chain Length:200
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS3 Pfam ID:PF00177

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS22E
Chain IDs:R (auth: AH), BB (auth: BH), LC (auth: CH), VD (auth: DH)
Chain Length:130
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU0 Pfam ID:PF00410

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS16E
Chain IDs:S (auth: AI), CB (auth: BI), MC (auth: CI), WD (auth: DI)
Chain Length:145
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT4 Pfam ID:PF00380

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS20E
Chain IDs:T (auth: AJ), DB (auth: BJ), NC (auth: CJ), XD (auth: DJ)
Chain Length:120
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT8 Pfam ID:PF00338

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS14E
Chain IDs:U (auth: AK), EB (auth: BK), OC (auth: CK), YD (auth: DK)
Chain Length:151
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT2 Pfam ID:PF00411

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN S12
Chain IDs:V (auth: AL), FB (auth: BL), PC (auth: CL), ZD (auth: DL)
Chain Length:142
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:P06147 Pfam ID:PF00164

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS18E
Chain IDs:W (auth: AM), GB (auth: BM), QC (auth: CM), AE (auth: DM)
Chain Length:155
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT6 Pfam ID:PF00416

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS29E
Chain IDs:X (auth: AN), HB (auth: BN), RC (auth: CN), BE (auth: DN)
Chain Length:55
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU7 Pfam ID:PF00253

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS13E
Chain IDs:Y (auth: AO), IB (auth: BO), SC (auth: CO), CE (auth: DO)
Chain Length:153
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT1 Pfam ID:PF00312, PF08069

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN S24
Chain IDs:Z (auth: AP), JB (auth: BP), TC (auth: CP), DE (auth: DP)
Chain Length:149
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU2 Pfam ID:PF01282

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS11E
Chain IDs:AA (auth: AQ), KB (auth: BQ), UC (auth: CQ), EE (auth: DQ)
Chain Length:157
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS9 Pfam ID:PF00366, PF16205

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RACK1
Chain IDs:BA (auth: AR), LB (auth: BR), VC (auth: CR), FE (auth: DR)
Chain Length:343
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBV0 Pfam ID:PF00400

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS15E
Chain IDs:CA (auth: AS), MB (auth: BS), WC (auth: CS), GE (auth: DS)
Chain Length:144
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT3 Pfam ID:PF00203

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS19E
Chain IDs:DA (auth: AT), NB (auth: BT), XC (auth: CT), HE (auth: DT)
Chain Length:155
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT7 Pfam ID:PF01090

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS12E
Chain IDs:EA (auth: AU), OB (auth: BU), YC (auth: CU), IE (auth: DU)
Chain Length:126
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT0 Pfam ID:PF01248

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS17E
Chain IDs:FA (auth: AV), PB (auth: BV), ZC (auth: CV), JE (auth: DV)
Chain Length:130
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT5 Pfam ID:PF00833

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS4E
Chain IDs:GA (auth: AW), QB (auth: BW), AD (auth: CW), KE (auth: DW)
Chain Length:259
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS2 Pfam ID:PF08071, PF00900, PF16121

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS30E
Chain IDs:HA (auth: AX), RB (auth: BX), BD (auth: CX), LE (auth: DX)
Chain Length:80
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBU8 Pfam ID:PF04758

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN S6
Chain IDs:IA (auth: AY), SB (auth: BY), CD (auth: CY), ME (auth: DY)
Chain Length:293
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBS4 Pfam ID:PF01092

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:40S RIBOSOMAL PROTEIN RPS21E
Chain IDs:JA (auth: AZ), TB (auth: BZ), DD (auth: CZ), NE (auth: DZ)
Chain Length:97
Number of Molecules:4
Biological Source:TETRAHYMENA THERMOPHILA

UniProt ID:E6PBT9 Pfam ID:PF01249

Ligand Molecules

Primary Citation

The crystal structure of the eukaryotic 40S ribosomal subunit in complex with eIF1 and eIF1A.

Weisser, M, Voigts-Hoffmann, F, Rabl, J, Leibundgut, M, Ban, N.Show

Nat. Struct. Mol. Biol. 20 1015 1017 (2013)

PMID: 23851459 DOI: 10.1038/nsmb.2622

Abstact

Eukaryotic translation initiation factors (eIFs) 1A and 1 are central players in the complex process of start-codon recognition. To improve mechanistic understanding of this process, we determined the crystal structure of the 40S ribosomal subunit in complex with eIF1A and eIF1 from Tetrahymena thermophila at a resolution of 3.7 Å. It reveals the positions of the two factors on the 40S and the conformational changes that accompany their binding.

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Protein

Chemical

Disease

Primary Citation of related structures

Abstact

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