ELECTRON MICROSCOPY

Sample

dengue virus type 2

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument LEICA EM GP
Cryogen Name ETHANE
Sample Vitrification Details GP2
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 2305
Reported Resolution (Å) 4.09
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details E-protein model was built using EMProt and Fab model was predicted from Alphafold. Realspace refined model from high resolution focused map was taken for rigid body. Map to model correlation is 0.84.
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 40
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 81000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 4.0.1
CTF CORRECTION cryoSPARC 4.6.0
MODEL FITTING UCSF Chimera 1.17
MODEL REFINEMENT PHENIX 1.21_5207
INITIAL EULER ASSIGNMENT cryoSPARC 4.6.0
FINAL EULER ASSIGNMENT cryoSPARC 4.6.0
CLASSIFICATION cryoSPARC 4.6.0
RECONSTRUCTION cryoSPARC 4.6.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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