ELECTRON MICROSCOPY

Sample

TASK2 in MSP1D1 lipid nanodisc at pH 8.5

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details 3uL sample, incubate 5 seconds, blot 3 seconds, blot force 1
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 78594
Reported Resolution (Å) 3.52
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol OTHER
Refinement Target ?
Overall B Value 151.61
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 1.00925
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model FEI TALOS ARCTICA
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 3.1 beta
IMAGE ACQUISITION SerialEM 3-6
CTF CORRECTION CTFFIND 4.1
MODEL FITTING Coot ?
MODEL REFINEMENT PHENIX ?
INITIAL EULER ASSIGNMENT RELION 3.1 beta
FINAL EULER ASSIGNMENT RELION 3.1 beta
CLASSIFICATION RELION 3.1 beta
RECONSTRUCTION RELION 3.1 beta
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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