ELECTRON MICROSCOPY

Sample

A149T VARIANT OF SERINE HYDROXYMETHYLTRANSFERASE 8 FROM SOYBEAN CULTIVAR ESSEX IN COMPLEX WITH PLP

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 71315
Reported Resolution (Å) 2.92
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target Correlation coefficient
Overall B Value 81.41
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON I (4k x 4k)
Electron Dose (electrons/Å2) 80
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 600
Maximum Defocus (nm) 2300
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 165000
Calibrated Magnification 98700
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v4.7.1
IMAGE ACQUISITION SerialEM ?
CTF CORRECTION cryoSPARC v4.7.1
MODEL FITTING UCSF ChimeraX 1.11.1
INITIAL EULER ASSIGNMENT cryoSPARC v4.7.1
FINAL EULER ASSIGNMENT cryoSPARC v4.7.1
CLASSIFICATION cryoSPARC v4.7.1
RECONSTRUCTION cryoSPARC v4.7.1
MODEL REFINEMENT PHENIX 1.21.2_5419
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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