ISDA: 9YNQ

Deposition Date 2025-10-11

Release Date 2026-04-08

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9YNQ

Keywords:

TRANSFERASE/DNA/RNA/INHIBITOR

Title:

Cryo-EM structure of Escherichia coli transcription initiation complex with GpA and des-hydroxy pseudouridimycin (des-hydroxy PUM)

Biological Source:

Source Organism(s):

Escherichia coli str. K-12 substr. MG1655 (Taxon ID: 511145)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.32 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:A, B
Chain Length:329
Number of Molecules:2
Biological Source:Escherichia coli str. K-12 substr. MG1655

UniProt ID:P0A7Z4 Pfam ID:PF01193, PF01000, PF03118 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoB
Chain IDs:C
Chain Length:1342
Number of Molecules:1
Biological Source:Escherichia coli str. K-12 substr. MG1655

UniProt ID:P0A8V2 Pfam ID:PF04563, PF04561, PF04565, PF10385, PF00562, PF04560 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoC
Chain IDs:D
Chain Length:1407
Number of Molecules:1
Biological Source:Escherichia coli str. K-12 substr. MG1655

UniProt ID:P0A8T7 Pfam ID:PF04997, PF00623, PF04983, PF05000, PF04998 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoZ
Chain IDs:E
Chain Length:91
Number of Molecules:1
Biological Source:Escherichia coli str. K-12 substr. MG1655

UniProt ID:P0A800 Pfam ID:PF01192 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:RNA polymerase sigma factor R
Gene (Uniprot):rpoD
Chain IDs:F
Chain Length:613
Number of Molecules:1
Biological Source:Escherichia coli str. K-12 substr. MG1655

UniProt ID:P00579 Pfam ID:PF03979, PF00140, PF04546, PF04542, PF04539, PF04545

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (27-MER)
Chain IDs:G
Chain Length:27
Number of Molecules:1
Biological Source:Escherichia coli str. K-12 substr. MG1655

Polymer Type:polydeoxyribonucleotide
Molecule:DNA
Chain IDs:H
Chain Length:21
Number of Molecules:1
Biological Source:Escherichia coli str. K-12 substr. MG1655

Polymer Type:polyribonucleotide
Molecule:RNA (5'-R(*GP*A)-3')
Chain IDs:I
Chain Length:2
Number of Molecules:1
Biological Source:Escherichia coli str. K-12 substr. MG1655

Ligand Molecules