ISDA: 9VJA

Deposition Date 2025-06-19

Release Date 2026-04-01

Last Version Date 2026-04-01

Entry Detail

PDB ID:

9VJA

Keywords:

DNA BINDING PROTEIN/DNA/RNA

Title:

Type I-A CRISPR integrase prespacer catching complex, State II

Biological Source:

Source Organism(s):

Enterococcus faecalis (Taxon ID: 1351)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.01 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Type II-A CRISPR-associated p
Chain IDs:G (auth: A), H (auth: B), I (auth: C), J (auth: D)
Chain Length:219
Number of Molecules:4
Biological Source:Enterococcus faecalis

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Chain IDs:A (auth: K), B (auth: L)
Chain Length:288
Number of Molecules:2
Biological Source:Enterococcus faecalis

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (51-MER)
Chain IDs:C (auth: N)
Chain Length:51
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Chain IDs:K (auth: S)
Chain Length:1337
Number of Molecules:1
Biological Source:Enterococcus faecalis

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (51-MER)
Chain IDs:D (auth: T)
Chain Length:83
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polyribonucleotide
Molecule:RNA (55-MER)
Chain IDs:E (auth: g)
Chain Length:55
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polyribonucleotide
Molecule:RNA (83-MER)
Chain IDs:F (auth: t)
Chain Length:83
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Structural basis for Cas9-directed spacer acquisition in type II-A CRISPR-Cas systems.

Li, Z, Li, Y, Kong, J, Wu, Q, Huang, P, Zhang, Y, Wu, W, Chen, M, Liu, Y, Lin, H, Hou, L, Liu, G, Zeng, T, He, Y, Hu, C, Yang, Z, Lu, M, Luo, M, Xiao, Y.Show

Mol.Cell 86 805 816.e4 (2026)

PMID: 41702404 DOI: 10.1016/j.molcel.2026.01.024

Primary Citation of related structures

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