9U3L image
Deposition Date 2025-03-18
Release Date 2026-03-25
Last Version Date 2026-04-08
Entry Detail
PDB ID:
9U3L
Keywords:
HYDROLASE
Title:
Substrate-engaged human 26S proteasome bound to midnolin with RPT2 at top of spiral staircase
Biological Source:
Source Organism(s):
Homo sapiens (Taxon ID: 9606)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
2.91 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
9U3L validation report
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Gene (Uniprot):PSMC2
Chain IDs:A
Chain Length:433
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P35998 Pfam ID:PF21236, PF00004, PF17862
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Gene (Uniprot):PSMC1
Chain IDs:B
Chain Length:377
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P62191 Pfam ID:PF16450, PF00004, PF17862
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Gene (Uniprot):PSMC5
Chain IDs:C
Chain Length:398
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P62195 Pfam ID:PF16450, PF00004, PF17862
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Gene (Uniprot):PSMC4
Chain IDs:D
Chain Length:418
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P43686 Pfam ID:PF16450, PF00004, PF17862
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome 26S subunit, ATPas
Gene (Uniprot):PSMC6
Chain IDs:E
Chain Length:70
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:A0A087X2I1 Pfam ID:PF16450, PF00004, PF17862
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Gene (Uniprot):PSMC3
Chain IDs:RA (auth: F)
Chain Length:439
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P17980 Pfam ID:PF16450, PF00004, PF17862
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Gene (Uniprot):PSMA6
Chain IDs:F (auth: G), BA (auth: g)
Chain Length:245
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P60900 Pfam ID:PF10584, PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Gene (Uniprot):PSMA2
Chain IDs:G (auth: H), CA (auth: h)
Chain Length:233
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P25787 Pfam ID:PF10584, PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Gene (Uniprot):PSMA4
Chain IDs:H (auth: I), DA (auth: i)
Chain Length:260
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P25789 Pfam ID:PF10584, PF00227 EC:3.4.25.1
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Gene (Uniprot):PSMA7
Chain IDs:I (auth: J), EA (auth: j)
Chain Length:247
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:O14818 Pfam ID:PF10584, PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Gene (Uniprot):PSMA5
Chain IDs:SA (auth: K), TA (auth: k)
Chain Length:240
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P28066 Pfam ID:PF10584, PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Isoform Long of Proteasome su
Gene (Uniprot):PSMA1
Chain IDs:J (auth: L), FA (auth: l)
Chain Length:268
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P25786 Pfam ID:PF10584, PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Gene (Uniprot):PSMA3
Chain IDs:K (auth: M), GA (auth: m)
Chain Length:254
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P25788 Pfam ID:PF10584, PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Gene (Uniprot):PSMB6
Chain IDs:L (auth: N), HA (auth: n)
Chain Length:238
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P28072 Pfam ID:PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Gene (Uniprot):PSMB7
Chain IDs:M (auth: O), IA (auth: o)
Chain Length:276
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:Q99436 Pfam ID:PF00227, PF12465
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Gene (Uniprot):PSMB3
Chain IDs:N (auth: P), JA (auth: p)
Chain Length:204
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P49720 Pfam ID:PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Gene (Uniprot):PSMB2
Chain IDs:O (auth: Q), KA (auth: q)
Chain Length:201
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P49721 Pfam ID:PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Gene (Uniprot):PSMB5
Chain IDs:P (auth: R), LA (auth: r)
Chain Length:262
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P28074 Pfam ID:PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Gene (Uniprot):PSMB1
Chain IDs:Q (auth: S), MA (auth: s)
Chain Length:240
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P20618 Pfam ID:PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Gene (Uniprot):PSMB4
Chain IDs:R (auth: T), NA (auth: t)
Chain Length:263
Number of Molecules:2
Biological Source:Homo sapiens
UniProt ID:P28070 Pfam ID:PF00227
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD1
Chain IDs:PA (auth: U)
Chain Length:953
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:Q99460 Pfam ID:PF21505, PF01851, PF13646, PF18004 EC:5.6.1.5
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD3
Chain IDs:S (auth: V)
Chain Length:15
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:O43242 Pfam ID:PF25573, PF01399, PF08375
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD12
Chain IDs:T (auth: W)
Chain Length:456
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:O00232 Pfam ID:PF22241, PF01399, PF18098
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD11
Chain IDs:U (auth: X)
Chain Length:422
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:O00231 Pfam ID:PF18055, PF01399, PF18503
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD6
Chain IDs:V (auth: Y)
Chain Length:389
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:Q15008 Pfam ID:PF10602, PF01399, PF21154
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD7
Chain IDs:W (auth: Z)
Chain Length:324
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P51665 Pfam ID:PF01398, PF13012
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD13
Chain IDs:X (auth: a)
Chain Length:433
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:Q9UNM6 Pfam ID:PF22037, PF01399
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD4
Chain IDs:Y (auth: b)
Chain Length:377
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P55036 Pfam ID:PF13519, PF02809
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD14
Chain IDs:Z (auth: c)
Chain Length:398
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:O00487 Pfam ID:PF01398, PF23594
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD8
Chain IDs:AA (auth: d)
Chain Length:418
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P48556 Pfam ID:PF10075
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome complex subuni
Gene (Uniprot):SEM1
Chain IDs:QA (auth: e)
Chain Length:70
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P60896 Pfam ID:PF05160
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Gene (Uniprot):PSMD2
Chain IDs:OA (auth: u)
Chain Length:953
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:Q13200 Pfam ID:PF17781, PF01851, PF18051
Polymer Type:polypeptide(L)
Molecule:substrate peptide
Chain IDs:UA (auth: v)
Chain Length:15
Number of Molecules:1
Biological Source:Homo sapiens
Ligand Molecules
ADP
ATP
LDZ
MG
ZN
Primary Citation
Structural dynamics of the midnolin-proteasome during ubiquitin-independent substrate turnover.
Nat Commun 17 ? ? (2026)
PMID: 41896529 DOI: 10.1038/s41467-026-71002-0
Primary Citation of related structures
9U3L
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